Sqstm1 p62 antibody

Manufactured by Abcam

SQSTM1/p62 antibody is a primary antibody that specifically recognizes the SQSTM1/p62 protein. SQSTM1/p62 is a multifunctional protein that plays a role in various cellular processes, including autophagy, oxidative stress response, and signal transduction.

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Lab products found in correlation

Foxo3 antibody, by Thermo Fisher Scientific (2 mentions) Lc3b d11 xp antibody, by Cell Signaling Technology (2 mentions) Ptch1 antibody, by Abcam (2 mentions) Bafilomycin a1, by Abcam (1 mentions) Beclin 1, by Cell Signaling Technology (1 mentions) Lc3b antibody, by Abcam (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions) Bcl 2, by Cell Signaling Technology (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Mcf 10a, by American Type Culture Collection (1 mentions) Mda mb 468, by American Type Culture Collection (1 mentions) Caspase 3, by Abcam (1 mentions) C myc, by Abcam (1 mentions) Hoechst 33258, by Solarbio (1 mentions)

Close spelling variants of this product

Mouse anti-SQSTM1/p62 antibody Anti-SQSTM1/P62 recombinant rabbit monoclonal antibody Anti-SQSTM1/p62 antibody SQSTM1/p62 mouse monoclonal antibody SQSTM1/p62 P62 antibody

3 protocols using sqstm1 p62 antibody

Protein Expression Analysis by Western Blot

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Protein samples were extracted with RIPA buffer. The extracted total protein was separated by SDS-PAGE and transferred to PVDF membranes. Following incubation in blocking buffer for 1 h at room temperature, the membrane was incubated at 4°C overnight with Ptch1 antibody (Abcam, ab109096), Foxo3 antibody (Thermo Fisher Scientific, FA1-14171), SQSTM1/p62 antibody (Abcam, ab56416), and LC3B (D11) XP antibody (Cell Signaling Technology, 3868S). The results were visualized using Beyo ECL Plus substrate and exposed to X-ray film. β-actin was used for normalization.

Tang S., Wang Y., Xie G., Li W., Chen Y., Liang J., Liu P., Song F, & Zhou J. (2020). Regulation of Ptch1 by miR-342-5p and FoxO3 Induced Autophagy Involved in Renal Fibrosis. Frontiers in Bioengineering and Biotechnology, 8, 583318.

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Autophagy regulation in breast cancer cells

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MDA-MB-231, MDA-MB-468, and MCF-10A cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). MDA-MB-231 and MCF-10A cells were cultured in DMEM with 10% fetal bovine serum, while MDA-MB-468 cells were cultured in L-15 with 10% fetal bovine serum and incubated with 5% CO₂. Antibodies used in this study were as follows: BRD4 (Cell Signaling Technology, no. 13440S), p-BRD4^S492 (EMD Millipore, ABE no. 1451), c-Myc (Abcam, ab56), LC3B antibody (Abcam, ab51520), SQSTM1/p62 antibody (Abcam, ab56416), CK2α (Cell Signaling Technology, no. 2656S), p-AKT^S129 (Abcam, 133458), GAPDH (Cell Signaling Technology, no. 5174), beclin-1 (Cell Signaling Technology, no. 3495), Bax (Cell Signaling Technology, no. 5023), Bcl-2 (Cell Signaling Technology, no. 2870), caspase-3 (Abcam, ab13847). MTT (M2128), 3-MA (M9281), and Hoechst 33258 were purchased from Solarbio. Bafilomycin A1 (ab120497) was purchased from Abcam.

Kandori H., Kinoshita N., Yamazaki Y., Maeda A., Shichida Y., Needleman R., Lanyi J.K., Bizounok M., Herzfeld J., Raap J, & Lugtenburg J. (2000). Local and distant protein structural changes on photoisomerization of the retinal in bacteriorhodopsin. Proceedings of the National Academy of Sciences of the United States of America, 97(9), 4643-4648.

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Western Blot Analysis of Protein Markers

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Protein samples were extracted with RIPA buffer. The extracted total protein was separated by SDS-PAGE and transferred to PVDF membranes. Following incubation in blocking buffer for 1 h at room temperature, the membrane was incubated at 4°C overnight with Ptch1 antibody (Abcam, ab109096), Foxo3 antibody (Thermo, FA1-14171), SQSTM1 / p62 antibody (Abcam, ab56416), and LC3B (D11) XP antibody (Cell Signaling Technology, 3868S). The results were visualized using Beyo ECL Plus substrate and exposed to X-ray lm. β-actin was used for normalization.

Tang S., Wang Y., Xie G., Li W., Chen Y., Liang J., Liu P., Song F., & Zhou J. (2020). Negative Regulation of Ptch1 by miR-342-5p and FoxO3 Induced Autophagy Involved in Renal Fibrosis.

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