Bisbenzamide h 33 342 trihydrochloride hoechst 33342

HeLa cervical cancer cells were purchased from Highveld Biological, Johannesburg, South Africa. RPMI 1640 cell culture medium and fetal bovine serum was purchased from GE Healthcare Life Sciences (Logan, UT, USA). Trypsin-EDTA, Dulbecco’s phosphate buffered saline (DPBS) with Ca²⁺ and Mg²⁺ and Dulbecco’s phosphate buffered saline (DPBS) without Ca²⁺ and Mg²⁺ were purchased from Lonza (Wakersville, MA, USA). Trypan blue, bisBenzamide H 33,342 trihydrochloride (Hoechst 33342), cisplatin, penicillin/streptomycin and bovine serum albumin fraction V were purchased from Sigma-Aldrich (St. Louis, MI, USA). NucRed^TM Live 647, CellRox^® Orange reagent, Lysotracker™ Deep Red and Tetramethylrhodamine ethyl ester (TMRE) were purchased from Molecular Probes^®-Life Technologies-Thermo Fisher Scientific (Logan, UT, USA). Annexin V-FITC/PI kit was purchased from MACS Miltenyi Biotec (Cologne, Germany). Cleaved caspase 3 (Asp175) (D3E9) Rabbit mAb, Cleaved caspase 8 (Asp391) (18C8) Rabbit mAb, Anti-rabbit IgG (H+L), F(ab’)2 fragment (Alexa fluor^® 647 conjugate), Anti-rabbit IgG (H+L), F(ab’)2 fragment (Alexa fluor^® 488 conjugate) and Phosphorylated Histone H3 were purchased from Cell Signaling Technology (Danvers, MA, USA).