Proteins were resolved by SDS-PAGE and transferred to nitrocellulose membrane (Whatman). Membranes were washed and incubated with antibodies as described previously11 (link). Briefly, membranes were blocked with blocking buffer (Sigma-Aldrich) for 1 h and were incubated with appropriate primary antibodies diluted in blocking buffer overnight at 4 °C. After three 5-min washes, membranes were incubated with secondary antibodies diluted in blocking buffer for 45 min in the dark. Secondary antibodies used were donkey Alexa Fluor 680-conjugated anti-goat IgG or anti-mouse IgG (Life Technologies) and donkey IRDye 800-conjugated anti-mouse IgG (Rockland Immunochemicals). Membranes were washed in the dark and scanned using the Odyssey infrared imaging system (LI-COR).
Donkey alexa fluor 680 conjugated anti goat igg or anti mouse igg
Manufactured by Thermo Fisher Scientific
Donkey Alexa Fluor 680-conjugated anti-goat IgG or anti-mouse IgG is a secondary antibody used in immunoassays and imaging applications. It is generated in donkeys and conjugated to Alexa Fluor 680, a fluorescent dye that can be detected using appropriate instrumentation.
Automatically generated - may contain errors
2 protocols using donkey alexa fluor 680 conjugated anti goat igg or anti mouse igg
1
Western Blot Protein Detection Protocol
2
Western Blot Protein Detection Protocol
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Proteins were resolved by SDS-PAGE and transferred to nitrocellulose membrane (Whatman). Membranes were washed and incubated with antibodies as described previously11 (link). Briefly, membranes were blocked with blocking buffer (Sigma-Aldrich) for 1 h and were incubated with appropriate primary antibodies diluted in blocking buffer overnight at 4 °C. After three 5-min washes, membranes were incubated with secondary antibodies diluted in blocking buffer for 45 min in the dark. Secondary antibodies used were donkey Alexa Fluor 680-conjugated anti-goat IgG or anti-mouse IgG (Life Technologies) and donkey IRDye 800-conjugated anti-mouse IgG (Rockland Immunochemicals). Membranes were washed in the dark and scanned using the Odyssey infrared imaging system (LI-COR).
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