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2 protocols using gq 11

1

VEGFR2 and Semaphorin 3E Signaling

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The antibodies used for western blotting included phospho(p)-ERK1/2T-202;Y-204, total(t)-ERK1/2, pAktS473, tAkt, p-eNOSS1177, pVEGFR2Y1175, tVEGFR2 (all antibodies from Cell Signaling Technology), t-eNOS (BD Biosciences), p-VinculinY822 (AbCam), t-Vinculin (Sigma Aldrich), PI3K/P85 (Upstate), integrin αvβ3 (clone LM609, Merck), Shc (Abcam),VE-cadherin (Santa Cruz), PlxnD1 (Thermo Fisher Scientific and Abcam), Piezo1 (Abcam), Gq/11 (Santa Cruz Biotechnology), Src (Upstate)
The inhibitors used in the study included VEGFR2 tyrosine kinase inhibitor SU1498 (Sigma Aldrich). Recombinant Semaphorin 3E was purchased from R&D Systems (Bio-techne, Minneapolis, MN, USA) and used at 10 μM. NRP1 blocking antibody was purchased from R&D Systems and Sema3E blocking antibody was from Thermo Fisher Scientific USA.
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2

VEGFR2 and Semaphorin 3E Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibodies used for western blotting included phospho(p)-ERK1/2T-202;Y-204, total(t)-ERK1/2, pAktS473, tAkt, p-eNOSS1177, pVEGFR2Y1175, tVEGFR2 (all antibodies from Cell Signaling Technology), t-eNOS (BD Biosciences), p-VinculinY822 (AbCam), t-Vinculin (Sigma Aldrich), PI3K/P85 (Upstate), integrin αvβ3 (clone LM609, Merck), Shc (Abcam),VE-cadherin (Santa Cruz), PlxnD1 (Thermo Fisher Scientific and Abcam), Piezo1 (Abcam), Gq/11 (Santa Cruz Biotechnology), Src (Upstate)
The inhibitors used in the study included VEGFR2 tyrosine kinase inhibitor SU1498 (Sigma Aldrich). Recombinant Semaphorin 3E was purchased from R&D Systems (Bio-techne, Minneapolis, MN, USA) and used at 10 μM. NRP1 blocking antibody was purchased from R&D Systems and Sema3E blocking antibody was from Thermo Fisher Scientific USA.
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