Hplc prominence system rf10axl

Manufactured by Shimadzu

Sourced in Japan

The HPLC-Prominence System RF10AXL is a high-performance liquid chromatography (HPLC) system with a fluorescence detector. It is designed to analyze and quantify various chemical compounds in complex samples. The system includes a pump, an autosampler, a column oven, and a fluorescence detector, all of which are integrated to provide reliable and consistent performance.

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Lab products found in correlation

Rf 10axl, by Shimadzu (3 mentions) Dgu 20a5, by Shimadzu (3 mentions) Lc 20at, by Shimadzu (2 mentions) Ptfe filter, by Merck Group (2 mentions) Polytetrafluoroethylene filter, by Merck Group (1 mentions) Sil 20a, by Shimadzu (1 mentions)

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HPLC system (1016 citations) Prominence HPLC system (232 citations) Prominence HPLC (83 citations) Prominence system (67 citations)

3 protocols using hplc prominence system rf10axl

Phytochemical Analysis of Plant Extracts

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The qualitative and quantitative analysis of resident phytochemicals such as alkaloids, carbohydrates, flavonoids, glycosides, phenols, saponins, and tannins in all the fractions were carried out using standard methods, as described previously.[23 24 25 ] For high-performance liquid chromatography (HPLC) analyses of the fractions, stock solutions were prepared in mobile phase for the sample and standards including gallic acid, reserpine, catechin, tannic acid, and quercetin and filtered using 0.45 μm polytetrafluoroethylene filter (Millipore, Billerica, MA, USA). An HPLC-Prominence System RF10AXL (Shimadzu Corp., Kyoto, Japan) equipped with degasser (DGU-20A5), quaternary pump (LC-20AT), auto-sampler (SIL-20A), and detectors of reflective index (RID-10A), fluorescence (RF-10AXL), and diode array (SPD-M20A) was used to perform the analysis. Gradient elution of the samples was done with consecutive mobile phases of acetonitrile and 0.5 mM ammonium acetate in water, at a flow rate of 1 mL/min for 70 min through the column (ZIC^®-HILIC) at 25°C temperature. The detection was carried out at 254 nm. The injection volume was 20 μl, and the sample and standards were analyzed in triplicates.

Shendge A.K., Basu T., Chaudhuri D., Panja S, & Mandal N. (2017). In vitro Antioxidant and Antiproliferative Activities of Various Solvent Fractions from Clerodendrum viscosum Leaves. Pharmacognosy Magazine, 13(Suppl 2), S344-S353.

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Phytochemical Profiling of Plant Extracts

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The analysis of resident phytochemicals, including alkaloids, carbohydrates, flavonoids, glycosides, phenols, saponins, tannins, terpenoids, anthraquinones and triterpenoids, in the extract was completed using standard qualitative and quantitative methods as previously described [33 ,34 ]. For HPLC analysis, standard stock solutions (10 μg/ml) were prepared in mobile phase for PRME, purpurin, catechin, tannic acid, reserpine, methyl gallate and rutin. All the samples were filtered through a 0.45-μm polytetrafluoroethylene (PTFE) filter (Millipore) to remove any particulate matter. The analysis was performed using a HPLC-Prominence System RF10AXL (Shimadzu Corp.) equipped with a degasser (DGU-20A5), quaternary pump (LC-20AT), auto-sampler (SIL-20A) and detectors of reflective index (RID-10A), fluorescence (RF-10AXL) and diode array (SPD-M20A). A 20 μl aliquot of each sample and standard was injected and analyzed in triplicate. Gradient elution consecutive mobile phases of acetonitrile and 0.5 mM ammonium acetate in water at a flow rate of 1 ml/min for 65 min through the column (ZIC-HILIC) was maintained at 25°C. The detection was completed at 254 nm.

Ghate N.B., Chaudhuri D., Das A., Panja S, & Mandal N. (2015). An Antioxidant Extract of the Insectivorous Plant Drosera burmannii Vahl. Alleviates Iron-Induced Oxidative Stress and Hepatic Injury in Mice. PLoS ONE, 10(5), e0128221.

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HPLC Analysis of Phytocompounds

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For HPLC analysis, stock solutions (10 μg/ml) were prepared in the mobile phase for the sample and different standard phytocompounds. Samples were then filtered through 0.45 μm polytetrafluoroethylene (PTFE) filter (Millipore) to remove any particulate matter. Analysis was performed using a HPLC-Prominence System RF10AXL (Shimadzu Corp., Japan) equipped with degasser (DGU-20A₅), quaternary pump (LC-20AT), auto-sampler (SIL-20A) and detectors of Reflective Index (RID-10A), Fluorescence (RF-10AXL) and Diode Array (SPD-M20A). The injection volume used was 20 μl and the sample and standards were analyzed in triplicates. Gradient elution consecutive mobile phases of acetonitrile and 0.5 mM ammonium acetate in water, at a flow rate of 1 ml/min for 80 min through the column (ZIC®-HILIC) that was maintained at 25°C. The detection was carried out at 254 nm.

Chaudhuri D., Baban Ghate N., Deb S., Panja S., Sarkar R., Rout J, & Mandal N. (2014). Assessment of the phytochemical constituents and antioxidant activity of a bloom forming microalgae Euglena tuba. Biological Research, 47(1), 24.

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