Liblia hfabp latex agglutination turbidimetric immunoassay

The urine and blood samples were collected within 30 min of admission. The urine and blood samples were centrifuged within 5 min at 4°C and immediately frozen at −80°C until they were analysed. The serum levels of HFABP, hsTnT, NT‐proBNP, and BNP were measured on admission. In addition, the urine NGAL, LFABP, β2MG, NAG, and albumin excretion were also measured on admission. These urine and serum biomarkers were measured by the Special Reference Laboratory (SRL©, Tokyo, Japan). The level of urine LFABP was measured with an enzyme‐linked immunosorbent assay (ELISA) using a human LFABP ELISA Kit (Kyowa Medex Co., Tokyo, Japan). The level of urinary NGAL was measured using the NGAL ELISA Kit (R&D Systems, Inc., Minneapolis, MN). The serum HFABP was measured using a MARKIT‐M HFABP ELISA Kit until June 2012 and using a LIBLIA HFABP latex agglutination turbidimetric immunoassay from July 2012 (DS Pharma Biomedical, Osaka, Japan). The lower and upper limits of detection for the urinary NGAL concentration were 4 and 500 pg/mL, respectively, and the lower limit for the urine LFABP was 2.9 pg/mL.