Hepatic triglyceride levels were measured using the Triglyceride Liquid Reagents Kit (Pointe Scientific). For fecal triglyceride quantitation, fecal samples were mixed with phosphate-buffered saline (PBS) in a 1:40 dilution and centrifuged at 12,000 g for 5 min to separate solid particles. Triglyceride concentrations in supernatants were determined as previously described employing the Triglyceride Liquid Reagents Kit (Pointe Scientific) [13 (link)]. Hepatic cholesterol levels were measured using the Cholesterol Liquid Reagents Kit (Pointe Scientific). Total serum bile acids were measured using a Mouse Total Bile Acid kit (Crystal Chem). Plasma lipopolysaccharide (LPS) was quantitated using an ELISA Kit for Lipopolysaccharides (LPS) (Lifeome Biolabs). For determination of tissue hydroxyproline content, liver specimens (100 mg) were homogenized in 6 N HCl (3750–32; USABlueBook), using lysing matrix C tubes with the Mini-BeadBeater-96, and subsequently incubated at 110°C for 24 hours. The lysate was filtered using Whatman filter paper, grade 595 1/2 (WHA10311644; Sigma Aldrich). After incubation with chloramine T– (C9887; Sigma Aldrich) and Ehrlich’s perchloric acid solution (AC168760250; Thermo Fisher Scientific), samples were measured at 558 nm (VersaMax Microplate Reader; Molecular Devices LLC, Sunnyvale, CA).
Mouse total bile acid kit
Manufactured by Crystal Chem
Sourced in United States
The Mouse Total Bile Acid kit is a laboratory equipment product designed to measure the total bile acid levels in mouse samples. It provides a quantitative analysis of the total bile acid concentration.
Automatically generated - may contain errors
Lab products found in correlation
Triglyceride liquid reagents kit, by Horiba (4 mentions)
Cholesterol liquid reagents kit, by Horiba (3 mentions)
Chloramine t, by Merck Group (2 mentions)
Versamax microplate reader, by Molecular Devices (2 mentions)
Ehrlich s perchloric acid solution, by Thermo Fisher Scientific (2 mentions)
Whatman, by Cytiva (2 mentions)
Infinity alt kit, by Thermo Fisher Scientific (2 mentions)
Quantichrom alkaline phosphatase assay kit, by BioAssay Systems (1 mentions)
Ethanol assay kit, by Abcam (1 mentions)
4 protocols using mouse total bile acid kit
1
Measuring Hepatic and Fecal Lipids in Mice
2
Quantifying Liver and Bile Metabolites
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Levels of plasma ALT were measured using the Infinity ALT kit (Thermo Scientific, Waltham, MA). Plasma and hepatic triglyceride and cholesterol levels were measured using the Triglyceride Liquid Reagents Kit (Pointe Scientific, Canton, MI) and Cholesterol Liquid Reagents kit (Pointe Scientific), respectively. Plasma levels of leptin were measured using the mouse leptin enzyme-linked immunosorbent assay kit according to the manufacturer’s protocol (Crystal Chem, Elk Grove Village, IL). Total bile acids and the bile acid pool were quantified using a Mouse Total Bile Acid Kit (Crystal Chem) as described.59 (link) For the total bile acid pool, total liver bile acids, total gallbladder bile acids, and total bile acids from the small intestine and cecum, contents were measured and calculated per gram of body weight.
3
Quantifying Liver Biochemical Markers
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Serum levels of alanine aminotransferase (ALT) were measured at 340 nm using Infinity ALT kit (Thermo Scientific, San Diego, CA, USA). The assay is linear up to 450 U/L. Serum alkaline phosphatase (ALP) was measured using QuantiChrom Alkaline Phosphatase Assay Kit (BioAssay Systems, Hayward, CA, USA) at 405 nm at time 0 and time 4. Levels of ethanol were measured using the Ethanol Assay Kit (BioVision, Milpitas, CA, USA) at 570 nm. Hepatic triglyceride levels were measured using the Triglyceride Liquid Reagents Kit (Pointe Scientific, Canton, MI, USA). The procedure is linear up to 1000 mg/dL and was measured at 500 nm. Hepatic cholesterol levels were measured at 600 nm using the Cholesterol Liquid Reagents Set (Pointe Scientific, Canton, MI, USA). Serum lipopolysaccharides (LPS) was measured by ELISA (Lifeome Biolabs, Oceanside, CA, USA). The minimum detectable dose of LPS is 5.15 ng/mL. Total serum bile acids were measured using a Mouse Total Bile Acid kit (Crystal Chem, Elk Grove Village, IL, USA) at 540 nm.
4
Quantitative Analysis of Hepatic and Fecal Lipid Profiles
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Hepatic triglyceride levels were measured using the Triglyceride Liquid Reagents Kit (Pointe Scientific). For fecal triglyceride quantitation, fecal samples were mixed with phosphate-buffered saline (PBS) in a 1:40 dilution and centrifuged at 12,000 g for 5 min to separate solid particles. Triglyceride concentrations in supernatants were determined as previously described employing the Triglyceride Liquid Reagents Kit (Pointe Scientific) [13 (link)]. Hepatic cholesterol levels were measured using the Cholesterol Liquid Reagents Kit (Pointe Scientific). Total serum bile acids were measured using a Mouse Total Bile Acid kit (Crystal Chem). Plasma lipopolysaccharide (LPS) was quantitated using an ELISA Kit for Lipopolysaccharides (LPS) (Lifeome Biolabs). For determination of tissue hydroxyproline content, liver specimens (100 mg) were homogenized in 6 N HCl (3750‐32; USABlueBook), using lysing matrix C tubes with the Mini‐BeadBeater‐96, and subsequently incubated at 110 °C for 24 h. The lysate was filtered using Whatman filter paper, grade 595 1/2 (WHA10311644; Sigma-Aldrich). After incubation with chloramine T– (C9887; Sigma-Aldrich) and Ehrlich's perchloric acid solution (AC168760250; Thermo Fisher Scientific), samples were measured at 558 nm (VersaMax Microplate Reader; Molecular Devices LLC, Sunnyvale, CA).
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