C2 spectral confocal microscope

Manufactured by Nikon

The C2+ spectral confocal microscope is a high-performance imaging system designed for advanced research applications. It features a spectral detection system that allows for simultaneous capture of multiple fluorescence channels, enabling detailed visualization and analysis of biological samples. The C2+ offers superior optical performance, delivering high-resolution, high-contrast images with minimal photobleaching.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Spectral C2+ microscope C2 confocal microscope C2 confocal C2 microscope Confocal microscope

2 protocols using c2 spectral confocal microscope

Mitochondrial Membrane Potential Assay

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MCF7 cell lines were loaded with tetramethylrhodamine methyl ester (TMRM, 5 nM, Life Technologies) for 30 min at 37°C and 5% CO2. Hoechst 33342 (Life Technologies) was used as nuclear counterstain. Images were acquired using a Nikon C2+ spectral confocal microscope using the same settings for all experiments. TMRM fluorescence was quantified with ImageJ software.

Jaña F., Bustos G., Rivas J., Cruz P., Urra F., Basualto-Alarcón C., Sagredo E., Ríos M., Lovy A., Dong Z., Cerda O., Madesh M, & Cárdenas C. (2019). Complex I and II are Required for Normal Mitochondrial Ca2+ Homeostasis. Mitochondrion, 49, 73-82.

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Mitochondrial Membrane Potential Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were loaded with tetramethylrhodamine methyl ester (TMRM; Life Technologies; 5 nM) for 30 min at 37°C and 5% CO₂. Hoechst 33342 was used as a nuclear counterstain. Images were acquired using a Nikon C2+ spectral confocal microscope using the same settings for all experiments. TMRM fluorescence was quantified with ImageJ software.

Cardenas C., Lovy A., Silva-Pavez E., Urra F., Mizzoni C., Ahumada-Castro U., Bustos G., Jaňa F., Cruz P., Farias P., Mendoza E., Huerta H., Murgas P., Hunter M., Rios M., Cerda O., Georgakoudi I., Zakarian A., Molgó J, & Foskett J.K. (2020). Cancer cells with defective oxidative phosphorylation require endoplasmic reticulum–to–mitochondria Ca2+ transfer for survival. Science signaling, 13(640), eaay1212.

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