Bis p sulfonatophenyl phenylphosphine dihydrate dipotassium salt bspp

Gold (III) chloride (HAuCl4), sodium citrate tribasic, tannic acid, potassium carbonate, Bis(p-sulfonatophenyl)phenylphosphine dihydrate dipotassium salt (BSPP), hydroquinone, and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tween 20 was purchased from Tokyo Chemical Industry (Tokyo, Japan). Thiol-terminated metoxypolyethylene glycol (PEG-SH) (Sunbright ME-050SH, MW = 5 kDa) was purchased from NOF Corp. (Tokyo, Japan). Sonazoid was purchased from Daiichi Sankyo Co. Ltd. (Tokyo, Japan). bEND.3 mouse brain endothelial cell was provided by the RIKEN cell bank. Penicillin–streptomycin-amphotericin B and 20% formalin were purchased from Wako Pure Chemical Industries (Osaka, Japan). Fetal bovine serum (FBS) and ZO-1 rabbit polyclonal antibodies were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). TRITC-conjugated anti-rabbit IgG was purchased from Agilent Technologies Inc. (Santa Clara, CA, USA). DAPI was purchased from Dojindo (Kumamoto, Japan). A silver enhancement kit was purchased from BBI Solutions (Crumlin, UK).