Cell surface markers of ASCs and SHED were analyzed by flow cytometry. In detail, cells were identified as CD105, CD44, CD73, and CD90 positive cells and negative for CD45 expression. Standard labelling protocol was performed with the following antibodies fluorochrome-conjugated and isotypic controls: human CD105 PE (Invitrogen, Camarillo, CA, USA), CD73 FITC (kindly provided by Prof. Malavasi, University of Turin), CD44 FITC, CD45 PerCP, IgG1 PE and IgG2a PerCP (Miltenyi Biotech, Bergisch Gladbach, Germany), and IgG1 FITC-conjugated (Immunostep). As further control, unstained cells were also examined. About 105 events/sample were used for capture with CellQuest software. Data were analyzed with FlowLogic software (Miltenyi Biotech).
Human cd105 pe
Manufactured by Thermo Fisher Scientific
Sourced in United States
Human CD105 PE is a fluorescently-labeled monoclonal antibody that binds to the CD105 (Endoglin) cell surface antigen. CD105 is a transmembrane glycoprotein that is expressed on endothelial cells and is involved in angiogenesis. The PE (Phycoerythrin) fluorescent label allows for the detection and analysis of CD105-positive cells using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Cd45 percp, by Miltenyi Biotec (4 mentions)
Cd44 fitc, by Miltenyi Biotec (4 mentions)
Flowlogic software, by Miltenyi Biotec (3 mentions)
Igg1 pe and igg2a percp, by Miltenyi Biotec (2 mentions)
Cd271 apc, by Miltenyi Biotec (2 mentions)
Igg2a percp, by Miltenyi Biotec (2 mentions)
Igg1 apc, by Miltenyi Biotec (2 mentions)
Cd73 fitc, by Thermo Fisher Scientific (2 mentions)
Igg1 pe, by Miltenyi Biotec (2 mentions)
Cd3 percp, by Miltenyi Biotec (1 mentions)
Cd3 apc, by Miltenyi Biotec (1 mentions)
4 protocols using human cd105 pe
1
Flow Cytometry of ASCs and SHED
2
Characterization of ATD-MSCs by Flow Cytometry
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Cell surface markers of ATD-MSCs were analysed by flow cytometry on fresh SVF and on SVF derived after thawing of adipose tissue and its collagenase treatment. ATD-MSCs were identified as CD105, CD44, CD73, and CD271 positive cells and negative for CD45 expression. Standard labelling protocol was performed with the following antibodies fluorochrome-conjugated and isotypic controls: human CD105 PE (Invitrogen), CD73 FITC (kindly provided by Professor Malavasi, University of Turin), CD44 FITC, CD45 PerCP, CD3 PerCP, CD271 APC, IgG1 PE, IgG1 APC, and IgG2a PerCP (Miltenyi Biotec), and IgG1 FITC-conjugated (Immunostep). About 105 events/sample were used for capture with CellQuest software. All data were analysed with FlowJo software (Tree Star).
3
Mesenchymal Cell Surface Marker Analysis
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Mesenchymal cell surface markers were analysed by flow cytometry on fresh SVF and on cultured ASCs. A standard labelling protocol was performed with the following antibody fluorochrome-conjugated and isotypic controls: human CD105 PE (Invitrogen), CD73 FITC (kindly provided by Prof. Malavasi, University of Turin), CD44 FITC, CD45 PerCP, CD271 APC, IgG1 PE, IgG1 APC and IgG2a PerCP (Miltenyi Biotec), and IgG1 FITC conjugate (IMMUNOSTEP). About 105 events/sample were used for capture with CellQuest software. All data were analysed with Flowlogic software (Miltenyi Biotec).
4
Characterization of Mesenchymal Stem Cells
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The expression of typical MSCs markers was analyzed by flow cytometry. In detail, cells were identified as CD105-, CD44-, CD73- and CD90-positive, or CD45-, and CD3-negative.
Standard labeling protocol was performed with the following fluorochrome-conjugated antibodies and isotypic controls: human CD105 PE (Invitrogen, Camarillo, CA, USA), CD73 FITC (kindly provided by Prof. Malavasi, University of Turin), CD44 FITC, CD45 PerCP, CD3 APC, IgG1 PE and IgG2a PerCP (Miltenyi Biotech, Bergisch Gladbach, Germany), and FITC-conjugated IgG1 (Immunostep). As a further control, unstained cells were also examined. About 105 events/sample were used for capture with CellQuest software. Data were analyzed with FlowLogic software (Miltenyi Biotech).
Standard labeling protocol was performed with the following fluorochrome-conjugated antibodies and isotypic controls: human CD105 PE (Invitrogen, Camarillo, CA, USA), CD73 FITC (kindly provided by Prof. Malavasi, University of Turin), CD44 FITC, CD45 PerCP, CD3 APC, IgG1 PE and IgG2a PerCP (Miltenyi Biotech, Bergisch Gladbach, Germany), and FITC-conjugated IgG1 (Immunostep). As a further control, unstained cells were also examined. About 105 events/sample were used for capture with CellQuest software. Data were analyzed with FlowLogic software (Miltenyi Biotech).
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