Cxcr4 pe

Manufactured by R&D Systems

CXCR4 PE is a fluorescently-labeled monoclonal antibody that binds to the CXCR4 chemokine receptor. CXCR4 is a G-protein-coupled receptor that plays a role in cell migration and immune cell trafficking. The PE (phycoerythrin) fluorescent label allows for the detection and quantification of CXCR4-expressing cells by flow cytometry.

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Lab products found in correlation

Facscanto 2 flow cytometer, by BD (1 mentions) Stabilizing fixative, by BD (1 mentions) Cd66b pecy7, by BioLegend (1 mentions) Cd69 bv421, by BioLegend (1 mentions) Cd63 fitc, by BioLegend (1 mentions) Cd16 percp, by BioLegend (1 mentions) Cd62l apc cy7, by BioLegend (1 mentions) Facscanto 2, by BD (1 mentions)

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CXCR4 (29 citations)

2 protocols using cxcr4 pe

Granulocyte Profiling in Inflammatory Bowel Disease

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Blood granulocytes and single-cell suspensions obtained from digested biopsies of heathy donors and IBD patients were stained with the following antibodies CD66b PE-Cy7 (#305116 clone G10F5, 1/20 dilution, BioLegend), CD16 PerCP (#302028 clone 3G8, 1/20 dilution, BioLegend), CD62L APC-Cy7 (#304814 clone DREG-56, 1/20 dilution, BioLegend), CD69 BV421 (#310930 clone FN50, 1/20 dilution, BioLegend), CD193 FITC (#310720 clone 5E8, 1/20 dilution, BioLegend), CD63 FITC (#353005 clone H5C6, 1/20 dilution, BioLegend) and CXCR4 PE (# FAB170P clone 12G5, 1/10 dilution, R&D systems) and Zombie Aqua Fixable Viability Kit 1/1000 (#423101, BioLegend) for death cells. Cells were fixed using BD Stabilizing Fixative [BD], acquired using a BD FACSCanto II flow cytometer (BD) and analyzed with FlowJO software (version 10.6.1, BD).

Garrido-Trigo A., Corraliza A.M., Veny M., Dotti I., Melón-Ardanaz E., Rill A., Crowell H.L., Corbí Á., Gudiño V., Esteller M., Álvarez-Teubel I., Aguilar D., Masamunt M.C., Killingbeck E., Kim Y., Leon M., Visvanathan S., Marchese D., Caratù G., Martin-Cardona A., Esteve M., Panés J., Ricart E., Mereu E., Heyn H, & Salas A. (2023). Macrophage and neutrophil heterogeneity at single-cell spatial resolution in human inflammatory bowel disease. Nature Communications, 14, 4506.

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Immunophenotypic Analysis of AML Cell Lines

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For immunophenotypic analysis AML cell lines were plated at 4 × 10⁵ cells/mL into 6 well plates and, the day after, 1 × 10⁶ cells were collected and stained with the following fluorochrome-conjugated anti-human antibodies: CXCR4 (PE, R&D Systems) and CD18 (ITGB2, APC, BD Pharmingen). Staining was performed at 4°C for 20 minutes in the dark.
1 × 10⁵ events were acquired at flow cytometer (FACS Canto II, Becton Dickinson). Surface expression (percentage of positive cells) and MFI (mean fluorescent intensity) values were calculated for CXCR4 and ITGB2 with FlowJo software.

Migliavacca J., Percio S., Valsecchi R., Ferrero E., Spinelli A., Ponzoni M., Tresoldi C., Pattini L., Bernardi R, & Coltella N. (2016). Hypoxia inducible factor-1α regulates a pro-invasive phenotype in acute monocytic leukemia. Oncotarget, 7(33), 53540-53557.

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