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3 protocols using goat α rabbit alexa fluor 488

1

Immunofluorescence Labeling Antibodies

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Goat α-rabbit HRP (cat. no. 7074, Cell Signaling), horse α-mouse HRP (cat. no. 7076, Cell Signaling), goat α-rabbit Alexa Fluor 488 (cat. no. A11008, Invitrogen, Stockholm, Sweden), goat α-mouse Alexa Fluor 488 (cat. no. A11029, Invitrogen), donkey α-rabbit Alexa Flour 594 (cat. no. A21207, Invitrogen) and donkey α-mouse Alexa Fluor 594(cat. no. A21203, Invitrogen) were all used in this study.
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2

Antibody panel for DNA damage response proteins

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Primary antibodies: rabbit α-WRAP53-C2 (cat. no PA-2020-100, Innovagen AB, Sweden), mouse monoclonal α-WDR79 (clone 1F12, cat. no. H00055135-M04; Abnova), rabbit α-pWRAP53βS64 (cat. no PA-2230-100, Innovagen AB), mouse monoclonal α-WDR79 (clone 1F12, cat. no. H00055135-M04; Abnova), rabbit α-WRAP53 (cat. no 14761-1-AP, Proteintech), mouse α-γH2AX (cat. no 05-636, Millipore), rabbit α-γH2AX (cat. no 2577, Cell Signaling), rabbit α-H2AX (cat. no. ab11175, Abcam), mouse α-MDC1 (cat. no ab50003, abcam), mouse α-RNF8 (cat. no sc-271462, Santa Cruz Biotechnology), mouse RPA32/RPA2 [9H8] (cat. no ab2175, abcam), rabbit α-53BP1 (cat. no NB100-904, Novus Biologicals), mouse α-coilin (cat. no. sc-56298, Santa Cruz Biotechnology), mouse α-β-actin (cat. no A5441, Sigma), rabbit α-GFP (cat. no ab290, abcam), mouse α-Flag (cat. no F1804, Sigma-Aldrich), normal rabbit IgG (cat. no sc-2027, Santa Cruz Biotechnology) and normal mouse IgG (cat. no sc-2025, Santa Cruz Biotechnology).
Secondary antibodies: goat α-rabbit HRP (cat. no 7074, Cell Signal), horse α-mouse HRP (cat. no 7076, Cell Signal), goat α-rabbit Alexa Fluor 488 (cat. no A11008, Life technologies), goat α-mouse Alexa Fluor 488 (cat. no A11029, Life technologies) and donkey α-mouse Alexa Fluor 594 (cat. no A21203, Life technologies).
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3

Immunofluorescence Labeling Reagents

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Goat α-rabbit HRP (cat. no. 7074; Cell Signaling), horse α-mouse HRP (cat. no. 7076; Cell Signaling), goat α-rabbit Alexa Fluor 488 (cat. no. A11008; Invitrogen, Thermo Scientific), goat α-mouse Alexa Fluor 488 (cat. no. A11029, Invitrogen, Thermo Scientific), donkey α-rabbit Alexa Flour 594 (cat. no. A21207; Invitrogen) and donkey α-mouse Alexa Fluor 594 (cat. no. A21203; Invitrogen).
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