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Fragment analyzer automated ce

Manufactured by Agilent Technologies
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The Fragment Analyzer Automated CE is a capillary electrophoresis instrument designed for the separation and analysis of DNA, RNA, and protein fragments. It provides automated high-resolution separation and sensitive detection of nucleic acid and protein samples.

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Lab products found in correlation

Kapa stranded mrna seq kit, by Roche (1 mentions) Nextseq 500 system, by Illumina (1 mentions)

Spelling variants of this product

Fragment Analyzer (884 citations) Fragment Analyzer Automated CE System (57 citations) Fragment Analyzer CE (7 citations) 5200 Fragment Analyzer Automated CE System (4 citations) Fragment Analyzer Automated CE platform (2 citations) Agilent fragment analyzer automated CE system with the high sensitivity NGS fragment analysis 474 kit (2 citations)

2 protocols using fragment analyzer automated ce

1

Transcriptome Analysis via RNA-seq

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cDNA libraries for each sample were prepared with Kapa Stranded mRNA-seq kit (KAPA Biosystems, Wilmington, MA). Library quality and quantity were assessed via Fragment Analyzer Automated CE (Advanced Analytical, Evry Cedex, France) and Qubit (Thermo Fisher) systems, respectively. Libraries were subsequently subjected to 2 × 75 bp paired-end (PE75) sequencing on a NextSeq. 500 system (Illumina). All samples were pooled and sequenced on one lane. All library preparation and sequencing were performed by the Georgia Genomics Facility (Athens, GA).
Cui X., Marshall B., Shi N., Chen S.Y., Rekaya R, & Liu H.X. (2017). RNA-Seq analysis on chicken taste sensory organs: An ideal system to study organogenesis. Scientific Reports, 7, 9131.
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2

Genotyping SINE Insertions Using Fragment Analysis

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To genotype a large number of samples we used fragment length analyses by assessing the PCR product sizes on the Fragment Analyzer Automated CE (capillary gel electrophoresis) System [Advanced Analytical Technologies (AATI)]. We used the PROSize analytical software (AATI) to visually inspect the obtained gel images and classify the dogs as homozygous for the SINE (short interspersed nuclear elements) insertion (ins/ins, single band of ∼425 bp), heterozygous (wt/ins, two bands of ∼198 and ∼425 bp), or homozygous wild type (wt/wt, single band of ∼198 bp).
Mauri N., Kleiter M., Dietschi E., Leschnik M., Högler S., Wiedmer M., Dietrich J., Henke D., Steffen F., Schuller S., Gurtner C., Stokar-Regenscheit N., O’Toole D., Bilzer T., Herden C., Oevermann A., Jagannathan V, & Leeb T. (2017). A SINE Insertion in ATP1B2 in Belgian Shepherd Dogs Affected by Spongy Degeneration with Cerebellar Ataxia (SDCA2). G3: Genes|Genomes|Genetics, 7(8), 2729-2737.
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