Dubelcco s modified eagle medium dmem

Medium molecular weight chitosan (MMW-CHT; ref.448877; 75% degree of deacetylation calculated by ¹H NMR – Figure S1 - Bruker DRX 300 Avance at 300.13 MHz; CHT was dissolved in a solution containing 98% D₂O and 2% DCl (v/v)), alginic acid sodium salt (ref. A3249), glacial acetic acid and phosphate buffer saline (PBS) were purchased from Sigma-Aldrich, and used as received. Sodium chloride (NaCl) was purchased from LabChem, and sodium hydroxide (NaOH) from AkzoNobel. Deuterium oxide and deuterium chloride (20% in Deuterium oxide) were purchased from Acros. Amicon® Ultra 4 mL centrifuge tubes with Ultracell 100 membrane (100 kDa cutoff), were purchased from Merck Millipore. Mouse fibroblast cell lines L929 (ref. 85011425) were purchased from Sigma-Aldrich, and human adipose-derived mesenchymal stem cells (hASCs; ref. ATCC® PCS-500-011™) from ATCC. Cell culture media Dubelcco’s Modified Eagle Medium (DMEM) was purchased from Sigma-Aldrich (ref. D2902). Minimum Essential Medium Eagle-Alpha Modification (αMEM; ref. 12000014), fetal bovine serum (FBS; ref. 10270106), Alamar Blue reagent (ref. DAL1025), Calcein AM (ref. C3099) and propidium iodide (ref. P1304MP), TrypLe™ Express Enzyme (ref. 12605010) and penicillin/streptomycin solution (ref. 15140122) were acquired from ThermoScientific.