Plasma glucose was immediately measured by the glucose–oxidase technique (Analox GM8 Glucose Analyzed, Analox Instruments Ltd., Amblecote, Stourbridge, UK). Insulin measurements were performed by electrochemiluminescence on a COBAS e411 instrument (Roche, Indianapolis, IN, USA). Plasma GLP-1 and GIP were assessed using a Multiplex technique (Biorad Laboratories, Hercules, CA, USA).
Multiplex technique
Manufactured by Bio-Rad
Sourced in United States
Multiplex technique is a laboratory method that enables the simultaneous detection and quantification of multiple analytes or targets within a single sample. It allows for the efficient and high-throughput analysis of complex biological samples.
Automatically generated - may contain errors
2 protocols using multiplex technique
1
Plasma Glucose, Insulin, and Gut Hormones
2
Measurement of Metabolic Markers
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In the clinical study, plasma glucose was measured immediately by the glucose-oxidase technique (Beckman Glucose Analyzer II, Beckman Instruments, Fullerton, CA). Insulin and C-peptide measurements were performed by electrochemiluminescence (COBAS e411, Roche, Indianapolis, IN). Plasma glucagon was assessed using a Multiplex technique (Biorad Laboratories, Hercules, CA). 6,6-[ 2 H 2 ]glucose and [U- 13 C] glucose were measured by gas chromatography-mass spectrometry (GC-MS). Plasma FFA and triglycerides were assayed by standard spectrophotometric methods on a Synchron Clinical System CX4 (Beckman Instruments). An in-house radioimmunoassay was used for measuring the amount of insulin secreted into the media during perifusion experiments [29] .
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