Cell free amino acid mixture 15n

Manufactured by Merck Group

Sourced in United States

The Cell Free Amino Acid Mixture-15N is a lab equipment product that provides a mixture of 15N-labeled amino acids. This product is designed for use in cell-free protein synthesis applications, where the labeled amino acids can be incorporated into the synthesized proteins for analysis and research purposes.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Amino acid mixture Amino acids

3 protocols using cell free amino acid mixture 15n

Targeted Metabolomics Using Nano-DESI

Check if the same lab product or an alternative is used in the 5 most similar protocols

Solvents were HLPC-grade
methanol (MeOH) (Fisher Scientific), water from Milli-Q Plus, LC/MS-grade
acetonitrile (Fisher Chemical), and formic acid (Merck). The standards
used for quantitation were Cell Free Amino Acid Mixture-¹⁵N (Sigma-Aldrich), glucose-d₂, γ-aminobutyric
acid-d₂ (GABA-d₂), acetylcholine-d₉, oleic acid-d₉ (FA 18:1-d₉),
LPC 19:0, PC 11:0/11:0. Solvents for PA nano-DESI analysis were MeOH/H₂O (9:1) with 0.1% formic acid or ACN/MeOH (9:1) with 0.1%
formic acid (Table S1).

Marques C., Friedrich F., Liu L., Castoldi F., Pietrocola F, & Lanekoff I. (2023). Global and Spatial Metabolomics of Individual Cells Using a Tapered Pneumatically Assisted nano-DESI Probe. Journal of the American Society for Mass Spectrometry, 34(11), 2518-2524.

+ Open protocol

+ Expand

Metabolite Quantification in Insulin-Secreting Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Solvents for extraction
were HLPC-grade methanol (MeOH) (Fisher Scientific), Milli-Q water
(H₂O) from Milli-Q Plus, and formic acid (Merck). For quantification
the internal standards oleic acid-d₉ (FA 18:1-d₉), LPC 19:0, PC 11:0/11:0, GABA-d₂, Acetylcholine-d₉, Glucose-d₂, and Cell Free Amino Acid Mixture-¹⁵N (Sigma-Aldrich) were prepared in MeOH:H₂O (9:1)
with 0.1% formic acid (Tables S1 and S2). INS-1 cells were exposed to a solution contained 138 mM NaCl,
5.6 mM KCl, 1.2 mM MgCl₂, 2.6 mM CaCl₂, and
1, 3, or 20 mM d-glucose at pH 7.4 and room temperature.

Marques C., Liu L., Duncan K.D, & Lanekoff I. (2022). A Direct Infusion Probe for Rapid Metabolomics of Low-Volume Samples. Analytical Chemistry, 94(37), 12875-12883.

+ Open protocol

+ Expand

Synthesis and Characterization of Gold Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Trifluoroacetic acid (TFA, 99%), α-cyano-4-hydroxycinnamic acid (CHCA, 98%), cyclohexane (99.5%), l-serine (99%), l-aspartic acid (98%), cell free amino acid mixture-¹⁵N (98%, 767972-1 EA) and 1H,1H,2H,2H-perfluorodecanethiol (PFDT, 97%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). HAuCl₄·4H₂O (99.9%), acetonitrile (99.9%), ethanol (99.7%) and methanol (99.8%) were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). l-arginine (99%), l-valine (99%) and sodium citrate tribasic dihydrate (99%) were purchased from J&K Scientific (Beijing, China). Novec™ 7500 Engineered Fluid (HFE-7500) was purchased from Minnesota Mining and Manufacturing Corporation (Maplewood, MN, USA). Deionized (DI) water (18.2 MΩ cm) used in all experiments was obtained from a Milli-Q system (Millipore, Bedford, MA, USA). SEM images were collected on a Zeiss Gemini 300 scanning electronic microscopy (Carl Zeiss AG, Oberkochen, Germany). MALDI-TOF MS analysis was performed with a Bruker MicroFlex LRF mass spectrometer (Bruker, Bremen, Germany). Optical microscopic image was obtained with inverted microscope (Cewei Optoelectronic Technology Ltd, Shanghai, China).

Han X., Li D., Wang S., Lin Y., Liu Y., Lin L, & Qiao L. (2022). Serum amino acids quantification by plasmonic colloidosome-coupled MALDI-TOF MS for triple-negative breast cancer diagnosis. Materials Today Bio, 17, 100486.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!