Antibody to bcl 2

Gastrodin was purchased from Chengdu Herbpurify CO., LTD (Chengdu, China). NaF was from Sinopharm Chemical Reagent CO., LTD (Shanghai, China). Sodium chloride injection (0.9%) was obtained from Shenyang North Pharmaceutical Factory (Shenyang, China). Ether and medical anhydrous EtOH (ethanol) were purchased from Beijing Chemical Works (Beijing, China). The antibody against Bax, caspase‐3 and caspase‐9 was from Proteintech Co. Ltd (Wuhan, China). The antibody to Bcl‐2 was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The Catalase Assay Kit was from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Other chemicals were provided by Beyotime Biotechnology Co. (Jiangsu, China). Mice MC3T3‐E1 cells (ATCC, Manassas, VA, USA) were grown in alpha‐MEM media (Invitrogen, Carlsbad, CA, USA) containing 10% FBS, 1% penicillin and streptomycin (Sigma, Taufkirchen, Germany).

αldiginoside was obtained from S. divaricatus collected in Pintung County, Taiwan, in June, 2013 [16 (link)]. The 1D and 2D proton nuclear magnetic resonance (NMR) spectral data and mass spectra of αldiginoside were used for the structural identification and purity determination with reference to available literature data (Figures S1–S6) [47 (link)]. All other reagents were obtained from Sigma-Aldrich (St. Lois, MO, USA) unless otherwise noted. Test agents were dissolved in dimethyl sulfoxide (DMSO) and added to culture medium with a final DMSO concentration of 0.1%. Antibodies to PARP, ERK, phosphor-ERK^{Thr202/Tyr204}, phospho-p38^{Thr180/Tyr182}, p38, phospho-JAK2^{Tyr1007/Tyr1008}, JAK2, phospho-STAT3^Ser727, Bak, caspase-3, Mcl-1, cyclin E, phospho-CDC25C^Ser216, and CDC25C were obtained from Cell Signaling Technologies (Danvers, MA, USA). Antibody to caspase-8 was obtained from Millipore. Antibody to Bcl-2 was purchased from Santa Cruz Biotechnology (Paso Robles, CA, USA). Antibody to Bax was purchased from Abcam (Cambridge, UK). The anti-β-actin was purchased from Sigma-Aldrich (St. Louis, MO, USA). Plasmids expressing Mcl-1 (Myc-DDK-tagged) and empty vector (pCMV6-Entry) were purchased from OriGene Technology (Rockville, MD, USA).