Glucose c6h12o6

Calcium chloride bihydrate (CaCl₂·2H₂O), magnesium chloride hexahydrate (MgCl₂·6H₂O), and glucose (C₆H₁₂O₆) were purchased from Vetec (Darmstadt, Germany). Sodium bicarbonate (NaHCO₃) was purchased from Fmaia (Belo Horizonte, Brazil). Sodium chloride (NaCl) and potassium chloride (KCl) were purchased from Química Moderna (São Paulo, Brazil). Monosodium dihydrogen orthophosphate (NaH₂PO₄), sodium hydroxide (NaOH) and hydrochloric acid (HCl) were purchased from Nuclear (Brazil). These substances were employed in the physiological Tyrode solution for functional experiments. Carbamylcholine hydrochloride (CCh) was purchased from Merck (Darmstadt, Germany). Thiobarbituric acid, tetramethoxypropane and perchloric acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Carbogen mixture (95% O₂ and 5% CO₂) was obtained from White Martins (Danbury, CT, USA).

Calcium chloride dihydrate (CaCl₂·2H₂O), magnesium sulfate heptahydrate (MgSO₄·7H₂O), and glucose (C₆H₁₂O₆) were purchased from Vetec (Rio de Janeiro, RJ, Brazil). Sodium bicarbonate (NaHCO₃), sodium chloride (NaCl), and potassium chloride (KCl) were purchased from Nuclear (Porto Alegre, RS, Brazil). Monobasic potassium phosphate (KH₂PO₄), monobasic sodium phosphate (NaH₂PO₄), sodium hydroxide (NaOH), and hydrochloric acid (HCl) were purchased from Dinâmica (Diadema, SP, Brazil).
Phenylephrine (PHE) was purchased from Pfizer (USA). ACh, R-(-)-apomorphine, Nω-nitro-L-arginine methyl ester (L-NAME), indomethacin, tempol, apocinin, ethylenediamine tetraacetic acid (EDTA), MDA, and 1,1-diPHEnyl-2-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (Brazil). To obtain the stock solutions, substances used in the functional experiments were dissolved and diluted in distilled water (indomethacin and apocinin were dissolved in absolute alcohol 96° GL), being, respectively, kept at 4 or -20°C. The carbogen mixture (95% O₂ and 5% CO₂) was obtained from White Martins (Brazil). All substances were weighed on an analytical balance, GEHAKA model AG 200 (Sao Paulo, SP, Brazil).