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5 protocols using peg 1000

1

Cyanoacrylate Adhesive Monomer Comparison

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The used materials were negative photoresist (SU-8 2150) and developer (mr-Dev 600) from Micro resist technology GmbH, Berlin, Germany, polydimethyl siloxane (PDMS) (Sylgard 184) from Dow Corning GmbH, Wiesbaden, Germany, Hexane (CHROMASOLV, for HPLC, >97.0% (GC) from Sigma-Aldrich, Taufkirchen, Germany, dopamine hydrochloride from Alfa Aesar, Kandel, Germany, poly(ethylene glycol) (PEG) with an average molecular weight of Mw¯ 1000 (PEG1000) from Acros Organics, part of Thermo Fisher Scientific, Hennigsdorf, Germany, methyl cyanoacrylate (Loctite 493), ethyl cyanoacrylate (Sicomet 40) and methoxyethyl cyanoacrylate (Loctite 460) from Henkel AG & Co, Düsseldorf, Germany. Samples of n-butyl cyanoacrylate (Cyberbond 7000) and ethoxyethyl cyanoacrylate (Cyberbond 5005) were kindly donated by Cyberbond Europe GmbH, Wunstorf, Germany. Table 1 shows the chemical structure of used CA monomers.
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2

Sunitinib-Loaded Polymeric Nanoparticles

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Sunitinib malate was obtained from LC laboratories, USA. Poly(vinyl alcohol) (PVA; M w 30,000-70,000; 88% hydrolyzed), Tween 20, stannous octoate and dimethyl sulfoxide (DMSO) were obtained from Sigma Aldrich, Germany. Dichloromethane (DCM), acetonitrile and tetrahydrofuran (THF) were purchased from Biosolve (Valkenswaard, The Netherlands). PEG1000, 1,4-butanediol and 1,4 dioxane were purchased from Acros (Geel, Belgium), 1,4butanediisocyanate was purchased from Bayer (Leverkusen, Germany) and DL-lactide and L-lactide were purchased from Corbion Purac (Gorinchem, The Netherlands). Polystyrene standards were purchased from Agilent technologies (Santa Clara, USA). Fertilized white Leghorn eggs were obtained from Het Anker company (Ochten, The Netherlands).
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3

Surface Functionalization of NPN-O Membranes

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The native surface of the NPN-O membranes was first deposited with NHS-Diazirine through vacuum phase reaction[45 (link)] and further grafted with PEG1000 (#B22134, Alpha Aesar, MA, USA) largely as described previously.[22 (link)] The PEG reaction temperature (75 °C) was increased for the 5.4 mm × 5.4 mm chips, because it was found that the high temperature increased the solubility of the PEG and helped the reaction on the NPN surface.
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4

Preparation of Organic Reagents and Solvents

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All applied organic reagents and solvents were of reagent grade purity and were used without further purification, unless stated otherwise. The 1-Methyl-1-phenylethyl hydroperoxide (cumyl hydroperoxide, CHP) was purified by the method described elsewhere [21 (link)].
The 1-Bromobutane (>98%) and cyclohexane were distilled prior to being used for synthesis. PEG (polyethylene glycol) 200, PEG 600, and PEG 1000 were purchased from Alfa Aesar GmbH & Co KG (Karlsruhe, Germany). PEG MM (polyethylene glycol monomethyl ether) 350, PEG MM 550, PEG MM 1100, PEG MM 2000, PEG DM polyethylene glycol dimethyl ether) 500, and PEG DM 1000 were purchased from Fluka Chemie GmbH (Steinheim, Germany).
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5

Polyethylene Glycol Derivatives Characterization

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Three different polyethylene glycol derivatives with increasing molecular weight were purchased for the study. The nominal average molecular weight, the actual molecular weight range, the physical form of the derivates and the manufacturer are the following for the compounds: PEG 1000, 950–1050 MW, solid flaskes, (Alfa Aesar, Karlsruhe, Germany), PEG 1500, 1400–1600 MW, powder, (Molar Chemicals, Halásztelek, Hungary), PEG 2000, 1900–2200 MW, powder, (Merck Lifesciences, Budapest, Hungary). Ketoprofen (≥98%) was purchased from TCI (Zwijndrecht, Belgium).
All materials were stored under dry, cool, and dark conditions until the measurements and the sample preparation.
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