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Anti human sod2

Manufactured by Cell Signaling Technology
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Anti-human SOD2 is a laboratory reagent used for the detection and quantification of the SOD2 (Superoxide Dismutase 2) protein in human samples. It is a highly specific antibody that binds to the SOD2 protein, enabling its identification and measurement through various analytical techniques.

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Fv10i confocal laser scanning microscope, by Olympus (2 mentions)

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Anti-SOD2 (22 citations)

2 protocols using anti human sod2

1

Quantifying Mitochondrial SOD2 Levels

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To assess mitochondrial superoxide dismutase 2 (SOD2) levels, both spheroids and USC-SFM samples were fixed with 4% PFA for 30min, permeabilized by 0.2% Triton-X 100, and blocked by DAKO protein block. The cell samples were incubated in primary antibodies anti-human SOD2 (Cell Signaling Technology, USA, diluted 1:50, Danvers, MA) overnight at 4 °C. Secondary antibody (Goat anti-Mouse, Alexa Fluor−™ 647, Thermo Fisher Scientific, USA, diluted 1:200) was then applied for 2 h at RT. Cell nuclei were stained by DAPI and then observed by an Olympus FV10i® confocal laser scanning microscope.
Ding H., George S., Leng X.I., Ihnat M., Ma J.X., Jiang G., Margolis D., Dumond J, & Zhang Y. (2022). Silk fibers assisted long-term 3D culture of human primary urinary stem cells via inhibition of senescence-associated genes: Potential use in the assessment of chronic mitochondrial toxicity. Materials today. Advances, 15, 100261.
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2

Quantifying Mitochondrial SOD2 Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
To assess mitochondrial superoxide dismutase 2 (SOD2) levels, both spheroids and USC-SFM samples were fixed with 4% PFA for 30min, permeabilized by 0.2% Triton-X 100, and blocked by DAKO protein block. The cell samples were incubated in primary antibodies anti-human SOD2 (Cell Signaling Technology, USA, diluted 1:50, Danvers, MA) overnight at 4 °C. Secondary antibody (Goat anti-Mouse, Alexa Fluor−™ 647, Thermo Fisher Scientific, USA, diluted 1:200) was then applied for 2 h at RT. Cell nuclei were stained by DAPI and then observed by an Olympus FV10i® confocal laser scanning microscope.
Ding H., George S., Leng X.I., Ihnat M., Ma J.X., Jiang G., Margolis D., Dumond J, & Zhang Y. (2022). Silk fibers assisted long-term 3D culture of human primary urinary stem cells via inhibition of senescence-associated genes: Potential use in the assessment of chronic mitochondrial toxicity. Materials today. Advances, 15, 100261.
+ Open protocol
+ Expand

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