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Rest 2009 programme

Manufactured by Qiagen
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The REST 2009 programme is a laboratory equipment product offered by Qiagen. It is designed to support the analysis of gene expression patterns. The core function of the REST 2009 programme is to provide tools for the relative quantification of gene expression levels.

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Lab products found in correlation

Abi prism 7900 system, by Thermo Fisher Scientific (1 mentions) Taqman probe, by Thermo Fisher Scientific (1 mentions) Revertaid h minus reverse transcriptase kit, by Thermo Fisher Scientific (1 mentions)

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REST 2009 software (128 citations)

2 protocols using rest 2009 programme

1

Quantitative Gene Expression Analysis

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RNA (1 μg, n = 10 samples per group) was used to synthesise cDNA with the RevertAid H Minus Reverse Transcriptase kit (Fermentas, Loughborough, UK). Quantification of gene expression was performed using the ABI Prism 7900 system (Applied Biosystems, Foster City, CA, USA) and primers for SYBR Green or TaqMan probes (Applied Biosystems) (ESM Table 2). Fold changes in gene expression were calculated using the REST 2009 programme (Qiagen, Manchester, UK) and normalised against Ppia, Taf2 and Hmbs, identified as good internal controls from the microarray data.
Sandovici I., Hammerle C.M., Cooper W.N., Smith N.H., Tarry-Adkins J.L., Dunmore B.J., Bauer J., Andrews S.R., Yeo G.S., Ozanne S.E, & Constância M. (2015). Ageing is associated with molecular signatures of inflammation and type 2 diabetes in rat pancreatic islets. Diabetologia, 59, 502-511.
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2

qRT-PCR Data Statistical Analysis

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For qRT-PCR data statistical analysis was performed using the REST 2009 programme (Qiagen, Manchester, UK). All other statistical tests were performed using the GraphPad 5.04 software (http://www.graphpad.com). A p value of <0.05 was considered statistically significant.
Sandovici I., Hammerle C.M., Cooper W.N., Smith N.H., Tarry-Adkins J.L., Dunmore B.J., Bauer J., Andrews S.R., Yeo G.S., Ozanne S.E, & Constância M. (2015). Ageing is associated with molecular signatures of inflammation and type 2 diabetes in rat pancreatic islets. Diabetologia, 59, 502-511.
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