Cd62l pe cy5

Manufactured by BioLegend

CD62L-PE-Cy5 is a fluorochrome-conjugated monoclonal antibody that specifically binds to the cell surface marker CD62L, also known as L-selectin. This antibody can be used for the identification and enumeration of cells expressing CD62L by flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

CD62L (93 citations) CD62L-PE (16 citations) Anti-CD62L-PE-Cy5 (clone DREG-56; (2 citations)

2 protocols using cd62l pe cy5

CAR T Cell Phenotypic Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eight days after activation, CAR T cells were either maintained alone or cocultured with SKBR3 tumor cells for 8 and 24 h under control and pro-oxidative conditions. Then, CAR T cells were collected, fixed, and stained to assess: i) CAR construct expression, ii) exhaustion state, and iii) memory state. The following antibodies were used for staining: i) CAR construct expression, IgG-PE (Southern Biotech, 2042-09); ii) exhaustion state CD3-PE (BD Biosciences, 345765), CD4-V450 (BD Biosciences Biosciences, 560345), CD8-FITC (BD Biosciences, 555366), PD1-APC (BioLegend, 367406), TIM3-APC-Cy7 (BioLegend, 345026), and LAG3-PE-Cy7 (BioLegend, 369310); and iii) memory state CD4-AF700 (BioLegend, 317426), CD8-APC-Cy7 (BioLegend, 344714), CD45RA-APC (BioLegend, 304112), CD45RO-PerCP-Cy5.5 (BioLegend, 304222), CCR7-PE-Cy7 (BioLegend, G043H7), CD62L-PE-Cy5 (BioLegend, 304808), Granzyme B-FITC (BD, 558132), IFN-γ-BV621 (BioLegend, 502536), CD127-BV421(BioLegend, 351310), and CD57-PE (BioLegend, 359612) according to the manufacturer’s instructions.

Balta E., Janzen N., Kirchgessner H., Toufaki V., Orlik C., Liang J., Lairikyengbam D., Abken H., Niesler B., Müller-Decker K., Ruppert T, & Samstag Y. (2022). Expression of TRX1 optimizes the antitumor functions of human CAR T cells and confers resistance to a pro-oxidative tumor microenvironment. Frontiers in Immunology, 13, 1063313.

+ Open protocol

+ Expand

Multicolor Immunofluorescence and Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used for immunofluorescence were rat anti-mouse CD68 (Serotec), hamster anti-mouse CD11c (N418, Biolegend), rabbit polyclonal anti-Brucella LPS (gift from Edgardo Moreno, Universidad Nacional, Costa Rica).
Antibodies for flow cytometry were MHCII-Alexa700 (eBioscience), CD11b-eFluor 450 (eBioscience), Ly6C-PerCP-Cy5.5 (BD Bioscience), Ly6G-APC-Cy7 (Biolegend), CD11c-PE-Cy7 (Biolegend), F4/80-PE (Biolegend), CD86-FITC, CD8-Alexa700 (BD Bioscience), CD4-APC-eFluor780 (eBioscience), CD19-BV-605 (Biolegend), CD62L-PE-Cy5 (Biolegend), CD5-PE-Cy7 (Biolegend), and CD44-FITC (BD Bioscience). Live cells were distinguished by staining with LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life technologies).

von Bargen K., Gagnaire A., Arce-Gorvel V., de Bovis B., Baudimont F., Chasson L., Bosilkovski M., Papadopoulos A., Martirosyan A., Henri S., Mège J.L., Malissen B, & Gorvel J.P. (2015). Cervical Lymph Nodes as a Selective Niche for Brucella during Oral Infections. PLoS ONE, 10(4), e0121790.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!