The final products of cGel-Mel and rGel-Mel were examined by SDS-PAGE, and western blot assay was further carried out using mouse anti-6×His tag antibody (Abcam, Cambridge, MA) to confirm the expression of rGel-Mel, following the previously reported procedures (24 (link), 25 (link)). The final cGel-Mel and rGel-Mel products were quantified by BCA assay, and the purity was determined by densitometry analysis (ImageJ software, National Institutes of Health, Bethesda, MD).
Mouse anti 6 his tag antibody
Manufactured by Abcam
Sourced in China
The Mouse anti-6xHis tag antibody is a monoclonal antibody that specifically binds to the 6x histidine (6xHis) tag, a commonly used protein tag for the purification and detection of recombinant proteins. This antibody can be used in various applications, such as Western blotting, immunoprecipitation, and ELISA, to detect and identify proteins with a 6xHis tag.
Automatically generated - may contain errors
2 protocols using mouse anti 6 his tag antibody
1
Characterization of cGel-Mel and rGel-Mel
2
Immunoblotting Analysis of Ats-1 Protein
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Immunoblotting analysis of the Ats-1 protein was performed through SDS-PAGE (CWBIO, Beijing, China) using mouse anti-6 × His-tag antibody (Abcam, Cambridge, MA, USA) (1:1000) as the primary antibody. Goat Anti-mouse IgG H&L (Abcam, Cambridge, MA, USA) (1:3000) was implemented as the secondary antibody, and a SuperSignal West Femto Trial Kit (ThermoFisher, Waltham, MA, USA) was utilized for color development. Actin was utilized as a control. Mouse Anti-β-actin Monoclonal Antibody (Sino Biological, Beijing, China) (1:1000) was used as the primary antibody and Goat Anti-mouse IgG H&L (Abcam, Cambridge, MA, USA) (1:3000) was used as the secondary antibody in our research. And the blots were cut prior to hybridisation with antibodies during blotting.
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