Atropine sulphate, Accustain formalin solution (10%, neutral buffered), dimethyl sulfoxide, ethanol, phosphate buffered saline (PBS, pH 7.4) tablets and Inactin (thiobutabarbital sodium) were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Sodium chloride was purchased from Merck KGaA (Darmstadt, Germany). 5‐FU Teva (solution for injection, 50 mg/ml), IRI Actavis (solution for infusion, 20 mg/ml) and MTX Ebewe (solution for injection, 100 mg/ml) were purchased from Apoteket AB (Stockholm, Sweden). DOX HCl and IDA HCl were purchased from Toronto Research Chemicals (Toronto, Canada). Transferrin Ki67 antibody (ab16667), horseradish peroxidase ‐ DAB detection IHC kit (ab64261) and TUNEL Assay Kit ‐ HRP‐DAB (ab206386) were purchased from Abcam, Cambridge, UK.
Tunel assay kit hrp dab ab206386
Manufactured by Abcam
Sourced in United Kingdom, Canada
The TUNEL Assay Kit - HRP-DAB (ab206386) is a laboratory tool designed to detect and quantify apoptosis, a form of programmed cell death. The kit utilizes the TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick-End Labeling) method to label DNA fragmentation, a hallmark of apoptosis. It employs horseradish peroxidase (HRP) and 3,3'-diaminobenzidine (DAB) for colorimetric detection of the TUNEL-positive cells.
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2 protocols using tunel assay kit hrp dab ab206386
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Detailed Reagents and Assays Protocol
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TUNEL Assay for Apoptosis Quantification
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A terminal deoxynucleotidyl transferase biotin-dUTP nick-end labelling kit (TUNEL Assay Kit—HRP-DAB-ab206386, Abcam Inc, Toronto, ON, Canada) was used according to the supplier’s instructions to identify apoptotic cells. The total number of positively stained apoptotic cells per sample cross-section (n = 4 per time point per group) was counted and the counting was cross-validated with ImageJ.
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