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Potassium nitrate

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Sourced in Spain

Potassium nitrate is a chemical compound with the formula KNO3. It is a crystalline salt that is commonly used in various industrial and laboratory applications. Potassium nitrate's core function is as an oxidizing agent, capable of supporting combustion and chemical reactions.

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5 protocols using potassium nitrate

1

Preparation of High-Purity Aqueous Solutions

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N-[tris(hydroxymethyl)methyl]glycine (Tricine, purity > 99 wt%), 2-[(2-hydroxy-1,1-bis(hydroxymethyl)ethyl)amino]ethane sulfonic acid (TES, purity > 99 wt%), 2-(N-morpholino) ethanesulfonic acid (MES, purity > 99 wt%), 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES, purity > 99.5 wt%), 2-(cyclohexylamino)ethanesulfonic acid (CHES, purity > 99 wt%), and [P4444][OH] (45 wt% in water), were purchased from Sigma–Aldrich. Sodium hydroxide pellets were supplied from Eka Chemicals. BSA (fraction V) was obtained from Acros Organics. Methanol (HPLC grade, purity > 99.9 wt%) was obtained from Fisher Scientific (UK). Acetonitrile (purity > 99.7 wt%) was supplied from Lab-Scan (Ireland). Purified water passed through a reverse osmosis and a Milli-Q plus 185 water purifying system was used in all experiments. Sodium nitrate (purity > 99.5 wt%) was obtained from Himedia Labs. Potassium nitrate (purity > 98.0), nitric acid (65 wt%), and potassium hydrogen phthalate (purity > 99.8) were purchased from Panreac (Barcelona, Spain).
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2

Synthesis of PDMS-based Composite Membranes

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Ultrapure water obtained using a Nanopure II system (Barnstead, NH, USA) was employed for the preparation and dilution of all the solutions. PDMS membranes were synthesized by using Sylgard® 184 Silicone Elastomer Kit (base and curing agent) obtained by Dow Corning (Midland, MI, USA). Tetraethyl orthosilicate (TEOS ≥ 99.0%), silicon dioxide nanoparticles (SiO2NPs, 99.5%, 5–15 nm particle size), N-(1-Naphthyl) ethylenediamine dihydrochloride (NEDD), 1-methyl-3-octylimidazolium hexafluorophosphate (OMIM PF6), Zinc nanopowder (ZnNPs, 99%, 40–60 nm particle size), hexadecyltrimethylammonium bromide (CTAB) were provided by Sigma-Aldrich (St. Louis, MO, USA). Sulfanilamide (SA) was obtained from Guinama (Valencia, Spain). Sodium Dodecyl Sulphate (SDS), potassium nitrate and anhydrous citric acid were provided by Panreac (Barcelona, Spain). Zn powder and sodium nitrite were purchased from Probus (Badalona, Spain). Silver nitrate was obtained from Scharlab (Barcelona, Spain).
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3

Optimizing Biomolecular Buffers for Analytical Applications

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PPG 400, choline hydroxide solution (46 wt% in methanol),
2-(N-morpholino) ethanesulfonic acid (MES, purity >99 wt%), 2-[(2-hydroxy-1,1-[bis(hydroxymethyl)ethyl)amino]ethane sulfonic acid
(TES, purity > 99 wt%),
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES, purity >99.5 wt%), N-[tris(hydroxymethyl)methyl]glycine (Tricine,
purity > 99 wt%), and 2-(cyclohexylamino)ethanesulfonic acid (CHES,
purity > 99 wt%), were supplied by Sigma–Aldrich Chemical Co.
Sodium hydroxide was purchased from Eka Chemicals. Methanol (HPLC grade, purity
> 99.9%) was supplied from Fisher Scientific and acetonitrile (purity
> 99.7%) was from Lab-Scan. Sodium nitrate (purity > 99.5%) was
acquired from Himedia Labs. Nitric acid (65 %), potassium nitrate (purity
> 98.0%), and potassium hydrogen phthalate (purity > 99.8%) were
obtained from Panreac. All solutions were prepared using ultra-pure water
(passed previously through a Milli-Q plus 185 system). Fresh eggs were purchased
in a local market. The molecular weight standards for SDS-PAGE, namely marker
molecular weight full-range, were acquired from VWR.
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4

Synthesis of Metal Complexes and Purification

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Clopyralid (99%), titanium (IV) butoxide (97%), ruthenium (II) chloride (RuCl3.xH2O, 99.9%) and zinc acetate (99%) were purchased from Sigma-Aldrich. Potassium nitrate and anhydrous sodium sulfate were from Panreac. Methanol and HPLC grade formic acid were obtained Sigma-Aldrich. All aqueous solutions were prepared with ultrapure water from a Millipore Mili-Q Gradient system (resistivity 18.2 MΩ cm).
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5

Electrochemical Detection of Paralytic Shellfish Toxins

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Sodium hydrogen phosphate and dihydrogen phosphate, aniline, tris(hydroxymethyl) aminomethane (BioPerformance Certified) were from Sigma Aldrich, ethanol, sodium hydroxide, hydrochloric acid, sulfuric acid, sodium nitrate, potassium nitrate and calcium nitrate were from Panreac, tetrahydrofuran (Chromasolv) was from Fisher. All reagents were p.a. (for analysis) unless stated otherwise. High molecular weight polyvinyl chloride (PVC), dibutyl phthalate (DBP), potassium tetrakis(4-chlorophenyl)borate (KTPB), tridodecylmethylammonium chloride (TDMACl) and ionophores were from Fluka. Screen-printed electrodes (SPE) with gold working and auxiliary electrodes and silver reference electrode were from DropSens (Spain). Ultrapure water produced by Merck Millipore Water System (18 MΩcm -1 ) was used for solution preparation and sensor washing.
Solutions of PSTs, namely saxitoxin (STX), decarbamoyl saxitoxin (dcSTX) and Nsulfocarbamoyl toxins gonyautoxin GTX5 and C1&C2, were certified reference material from the Institute for Marine Biosciences, National Research Council, Halifax, Canada. When working with PSTs, long sleeved lab coat and non-permeable nitrile or latex gloves should be used. Toxin containing waste should be decontaminated using a 10% solution of sodium hypochlorite during 30 minutes and disposed of down the drain with plenty of water.
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