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Nanowizard 4bio afm

Manufactured by Bruker
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Sourced in United States, Germany

The NanoWizard 4Bio AFM is a high-resolution atomic force microscope designed for biological applications. It provides nanoscale imaging and force spectroscopy capabilities for the study of samples in liquid and air environments.

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Lab products found in correlation

Cell tak, by Merck Group (1 mentions) Pfqnm lc a cal cantilever, by Bruker (1 mentions) Jpk bioafm, by Bruker (1 mentions) Data processing software, by Bruker (1 mentions) Inspect f50, by Thermo Fisher Scientific (1 mentions) Nsg01, by NT-MDT (1 mentions)

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NanoWizard AFM

4 protocols using nanowizard 4bio afm

1

Atomic Force Microscopy of Immobilized Cells

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Cells were immobilized onto clean glass slides using CellTak (Merck, Germany) and imaged in NanoWizard 4Bio AFM (Bruker, USA) using Quantitative Imaging mode under ambient temperature and buffered with 150 mM KCl and 10 mM Tris.Cl pH 7.5. PFQNM-LC-A-CAL cantilevers (Bruker, USA) with tip length of 17 µm were calibrated using the method by Sader et al. (62 (link)) yielding a spring constant k = 0.033 N·m−1 and a resonant frequency f0 = 39.8 kHz in air and f0 = 20.1 kHz in the buffer. The 6 × 6 µm2 topography images were scanned with a resolution of 256 × 256 pixels2 at a setpoint of 80 pN and the tip vertical speed was 83.3 µm s−1.
Tomasch J., Kopejtka K., Bílý T., Gardiner A.T., Gardian Z., Shivaramu S., Koblížek M, & Kaftan D. (2024). A photoheterotrophic bacterium from Iceland has adapted its photosynthetic machinery to the long days of polar summer. mSystems, 9(3), e01311-23.
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2

Measuring Cell Mechanical Properties via AFM

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To analyse the mechanical properties of the cells, AFM was used. The cells were trypsinized right before the experiment and seeded in a confocal imaging dish (VWR) to a density of 500,000 cells/mL. To prevent any changes in mechanical properties due to formation of stress fibres (involved in cell attachment), the mechanical properties were measured immediately after adding the cells to the dish (while still in suspension). The AFM instrument is a Nanowizard 4 BioAFM (JPK bioAFM, Bruker). The measurements were performed in a liquid environment using an in‐house‐made colloidal probe with the radius of 5 μm. To extract the Young's modulus, the obtained force curves were processed in the JPK DP software using an adjusted Hertz model for spherical probes.
Van der Meeren L., Verduijn J., Krysko D.V, & Skirtach A.G. (2023). High‐throughput mechano‐cytometry as a method to detect apoptosis, necroptosis, and ferroptosis. Cell Proliferation, 56(6), e13445.
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3

Elastic Modulus Mapping of GelMa Hydrogels

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Suspensions of 30Vf GelMa particles with filler were crosslinked in 6×6×1mm plastic molds glued down to glass coverslips. Shorter molds were used to limit light diffraction for the camera on the AFM’s microscope. The samples were fixed to the bottom of fluorodishes (Coherent, FD35) with 2-part rubber cement. The samples were then submerged in water until ready. All data was acquired with the JPK NanoWizard4 Bio-AFM with a spherical probe (2 µm diameter Borosilicate unmodified probe, Novascan). The tip spring constant was calibrated on glass in water prior to the experiment. Using contact-force microcopy mode, 36 force curves (6 µm approach at 0.5 µm per second) were taken per 10×10µm regions in different locations of the gel. A stitched optical image was taken to find particles and filler spaces between. The curves were loaded in the JPK Data Processing software to calculate the elastic modulus at each region. The following analysis steps were performed:
Molley T.G., Jalandhra G.K., Nemec S.R., Tiffany A.S., Patkunarajah A., Poole K., Harley B.A., Hung T.T, & Kilian K.A. (2021). Heterotypic tumor models through freeform printing into photostabilized granular microgels. Biomaterials science, 9(12), 4496-4509.
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4

Reversible Surface Wrinkles in PEDOT:PSS

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The reversible formation of surface wrinkles within the PEDOT:PSS thin films at different area strains was investigated using an atomic force microscope - AFM (NanoWizard 4 BioAFM, JPK Instruments AG, Germany) and a scanning electron microscope - SEM (Inspect F50, FEI, USA). The AFM images were taken with a cantilever probe (NSG01, NT-MDT, Russia) having a resonant frequency of around 150 kHz and a spring constant of 3.5 N/m. The SEM images were taken with an acceleration voltage of 2 kV.
Chen L., Ghilardi M., Busfield J.J, & Carpi F. (2019). Electrically tuning soft membranes to both a higher and a lower transparency. Scientific Reports, 9, 20125.
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