Cells were detached with Versene (Invitrogen) counted and resuspended in 100 µL phosphate buffered saline (PBS) containing 1% bovine serum albumin (BSA). Cells were blocked with PBS/1% BSA for 1 h on ice. Two micrograms of QUAD 3.0, EphA3 antibody (house made),9 (link) EphA2, EphB2 (R&D Systems), or IL-13RA2 (Biolegend) was added and incubated for 2 h on ice with occasional mixing. Cells incubated with isotype antibody served as controls. Cells were washed with PBS/1% BSA prior to the addition of Alexa-Fluor-labeled secondary antibodies. Cells were incubated on ice for an additional 1 h with occasional mixing. After washing with PBS/1% BSA, cells were postfixed with 10% buffered formalin. Cells were analyzed on an Accuri 6 flow cytometer. Data were analyzed using the FCS Express software (DeNovo Software).
Epha3
Manufactured by R&D Systems
Sourced in United States
EphA3 is a recombinant protein that functions as a receptor tyrosine kinase. It belongs to the Eph receptor family and is involved in various cellular processes, including cell-cell communication, cell adhesion, and cell migration.
Automatically generated - may contain errors
2 protocols using epha3
1
Antibody Staining and Flow Cytometric Analysis
2
Validating Novel EphA2 ELISA Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The novel in-house EphA2 ELISA system of the current study was verified by applying four different concentrations (250, 500, 1000, and 2000 pg/mL) of recombinant EphA2 (Cat. # 3035-A2; R&D Systems, Minneapolis, MN, USA) to determine whether the assay worked properly. The specificity of the novel EphA2 ELISA assay was examined next by demonstrating the absence of an increased signal after the addition of the same concentrations (250, 500, 1000, and 2000 pg/mL) of recombinant human EphA1 (Cat. # 7146-A2; R&D Systems, Minneapolis, MN, USA), EphA3 (Cat. # 6444-A3; R&D Systems, Minneapolis, MN, USA), or EphA4 (Cat. # 6827-A4; R&D Systems, Minneapolis, MN, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!