Mouse monoclonal anti‐FKBP51 antibody was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Rabbit polyclonal anti‐FKBP51, mouse monoclonal anti‐FLAG, and rabbit polyclonal anti‐StARD13 antibodies were obtained from Sigma‐Aldrich (St. Louis, MO, USA). Mouse monoclonal anti‐HALO antibody was obtained from Promega (Madison, WI, USA). Mouse monoclonal anti‐RhoA antibody was obtained from Cytoskeleton, Inc. (Denver, CO, USA). Mouse monoclonal anti‐cortactin antibody was obtained from Merck Millipore (Billerica, MA, USA). Rabbit polyclonal anti‐Smad2 and rabbit polyclonal anti‐Phospho‐Smad2 (Ser465/467) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Fluorescent secondary antibodies (Alexa Fluor 488) were obtained from Life Technologies (Carlsbad, CA, USA). To visualize the actin cytoskeleton, cells were stained with rhodamine phalloidin (Sigma‐Aldrich). Hoechst staining was used to detect nuclei.
Mouse monoclonal anti cortactin antibody
Manufactured by Merck Group
Sourced in United States
The mouse monoclonal anti-cortactin antibody is a laboratory tool used for the detection and study of the cortactin protein. Cortactin is an actin-binding protein involved in the regulation of the actin cytoskeleton. This antibody can be utilized in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to investigate the expression, localization, and interactions of cortactin.
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Lab products found in correlation
Mouse monoclonal anti rhoa antibody, by Cytoskeleton (1 mentions)
Rhodamine phalloidin, by Merck Group (1 mentions)
Mouse monoclonal anti flag, by Merck Group (1 mentions)
Phalloidin tetramethylrhodamine b isothiocyanate, by Merck Group (1 mentions)
Alexa fluor 488 labeled anti mouse antibody, by Thermo Fisher Scientific (1 mentions)
Lsm 710 confocal laser scanning microscope, by Zeiss (1 mentions)
2 protocols using mouse monoclonal anti cortactin antibody
1
Immunofluorescence Analysis of Cellular Proteins
2
Immunofluorescence Imaging of Endometrial Cancer Cells
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AN3CA, Nestin knockdown AN3CA, KLE, Nestin knockdown KLE, Ishikawa, and Nestin overexpressing Ishikawa cells endometrial cancer cells seeded on coverslips were fixed in 4% paraformaldehyde for 20 minutes at room temperature and permeabilized with phosphate buffered saline (PBS) containing 0.1% Triton X-100 (PBS-T) for 10 minutes, and subsequently blocked with PBS-T containing 5% normal goat serum for 1h. Cells were stained with mouse monoclonal anti-cortactin antibody (EMD Millipore) for 1h in PBS-T containing 1% normal goat serum and a secondary antibody Alexa Fluor® 488-labeled anti-mouse antibody (Invitrogen Life Technologies). To detect F-actin, cells were stained with Phalloidin–Tetramethylrhodamine B isothiocyanate (Sigma-Aldrich) for 20 minutes and the cell nuclei were stained with 1 μg/mL DAPI for 10 minutes. Fluorescent images of cells were captured using a LSM 710 laser scanning confocal microscope (Carl Zeiss Microscopy, LLC Thornwood NY).
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