Samples of fresh ruminal tissues were washed in a phosphate buffer (0.1 M, pH 7.4), put in plastic containers and fixed in a 10% buffered FA solution as pieces of tissue spread on flat polystyrene. The fixed material was processed using a series of reagents and embedded in Paraplast PLUS paraffin blocks (Leica, Buffalo Grove, USA), which were then cut with a rotary microtome into sections 3.5 μm thick. Slides with a paraffin section were automatically stained with hematoxylin and eosin (Varistain Gemini Thermo Scientific, Runcorn, UK). An Axio Lab. 1 microscope (Carl Zeiss, Jena, Germany) equipped with a Zeiss Axiocam ERc5s digital camera was used for histological evaluation. Photographs were analyzed and recorded using ZEN 2.3 (blue edition) software (Carl Zeiss Microscopy GmbH, 2011).
Axio lab 1 microscope
Manufactured by Zeiss
Sourced in Germany
The Axio Lab. 1 microscope is a high-performance optical instrument designed for routine laboratory applications. It features a sturdy, ergonomic construction and a range of optical components to enable clear, detailed observations. The microscope's core function is to magnify and display samples for analysis and examination.
Automatically generated - may contain errors
Lab products found in correlation
Axiocam erc5s digital camera, by Zeiss (2 mentions)
Lsm 700 confocal microscope, by Zeiss (2 mentions)
Zen 2.3 blue edition software, by Zeiss (1 mentions)
Quanta 250, by Thermo Fisher Scientific (1 mentions)
Eclipse e800 epifluorescence microscope, by Nikon (1 mentions)
Zen 2012 imaging software, by Zeiss (1 mentions)
Axiovision software, by Zeiss (1 mentions)
Erc5s digital camera, by Zeiss (1 mentions)
Zen 2, by Zeiss (1 mentions)
Paraplast plus, by Leica (1 mentions)
Zen 2.3 blue edition, by Zeiss (1 mentions)
5 protocols using axio lab 1 microscope
1
Histological Evaluation of Rumen Tissue
2
Microscopic Characterization of Streptomyces ghanaensis
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The mycelial morphology of S. ghanaensis in liquid-grown cultures was monitored using a Carl Zeiss Axiolab 1 microscope. For scanning electron microscopy (SEM), slices of S. ghanaensis lawns were cut off SFM agar plates and directly analyzed on a Quanta 250 environmental scanning electron microscope (ThermoFisher).
3
Multimodal Microscopy Imaging of Cells
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Images of H&E and IHC staining were taken by an Axio Lab 1 microscope using 10×, 20×, and 40× Zeiss A-Plan objectives and were captured using a Canon EOS 1000D camera and AxioVision software (Carl Zeiss). Images of IF staining and organoids were acquired on a Nikon ECLIPSE E800 epi-fluorescence microscope at 10×, 20×, and 40× Nikon Plan Fluor objectives using an QImaging RETGA EXi camera with QCapture software (QImaging). Fluorescence images for nuclear pore complex were collected using Zeiss LSM-700 confocal microscope with Zen 2012 Imaging Software.
4
Histological Examination of Urogenital Organs
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In postmortem examination, the reproductive and urinary organs were removed and fixed in neutral 10% formalin solution. Individual sections of the urogenital apparatus were prepared and macroscopically described. Samples of tissues were then taken from the ovaries, fallopian tubes, uterine horns, uterine body, cervix, vagina, ureters, and urethra. Paraffin sections (3 μm), stained with hematoxylin and eosin (H&E), were examined, and micrographs were taken using an Axio Lab.1 microscope (Carl Zeiss, Oberkochen, Germany) equipped with an ERc5s digital camera (Carl Zeiss, Oberkochen, Germany). Zen 2.3 software (blue edition; Carl Zeiss Microscopy, 2011) were used.
5
Histological Analysis of Rumen Tissue
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Samples of fresh tissues of rumen were washed in phosphate buffer solution (0.1 M, pH 7.4), put in a plastic container and xed in a 10% buffered FA solution as spread tissues pieces on at polystyrene. The xed material was processed with a series of reagents and embedded in para n blocks (Paraplast PLUS, Leica, Buffalo Grove, USA), which were then cut with a rotary microtome into 3.5 μm thick sections. Slides with a para n section were automatically stained with hematoxylin and eosin (HE, Varistain Gemini Thermo Scienti c, UK). Histological evaluation was performed using an Axio Lab. 1 microscope (Carl Zeiss) equipped with an Axiocam ERc5s digital camera (Carl Zeiss, Jena, Germany). Pictures were analyzed and recorded by computer software (ZEN 2.3 (blue edition), Carl Zeiss Microscopy GmbH, 2011).
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