Macroh2a

Manufactured by Abcam

MacroH2A is a histone variant that is involved in chromatin regulation. It plays a role in transcriptional repression and is associated with heterochromatin formation.

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Lab products found in correlation

Brca1, by Santa Cruz Biotechnology (2 mentions) Hrp linked anti rabbit igg, by Cell Signaling Technology (2 mentions) Hrp linked anti mouse igg, by Cell Signaling Technology (2 mentions) H2a z, by Cell Signaling Technology (2 mentions) γh2ax, by Merck Group (2 mentions) Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (2 mentions) Flag m2, by Merck Group (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Rap80, by Fortis Life Sciences (1 mentions) P chk1, by Cell Signaling Technology (1 mentions) Patm s1981, by Abcam (1 mentions) Rad18, by Cell Signaling Technology (1 mentions) β tubulin, by Abcam (1 mentions) Zmym3, by Abcam (1 mentions) Prpa2 s33, by Fortis Life Sciences (1 mentions) Alexa fluor 594 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Rad51, by Abcam (1 mentions) Alexa fluor 594 goat anti mouse antibody, by Thermo Fisher Scientific (1 mentions)

2 protocols using macroh2a

Antibody Panel for DNA Damage Response

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Primary antibodies used in this study were Flag M2 (Sigma, F1804), Myc (Santa Cruz Biotechnology, SC-40), H2AX (Millipore, 07-627), γH2AX (Millipore, 05-636), H2AZ (Cell Signaling, 2718S), macroH2A (Abcam, AB37264), H2A.Bbd (Millipore, 06-1319), ZMYM3 (Abcam, AB106626), ATM (Santa Cruz Biotechnology, SC-135663), pATM S1981 (Abcam, AB81292), β-tubulin (Abcam, AB6046), RAD51 (Abcam, AB88572), RAP80 (Bethyl Laboratories, A300-763A), ABRA1 (Abcam, AB139191), BRCA1 (Santa Cruz Biotechnology, SC-6954), HRP-linked anti-GST (Sigma, A7340), MBP (Abcam, AB9084), phospho-H3 S10 (Cell Signaling, 3377), 53BP1 (Novus Biologicals, NB100-304), RAD18 (Cell Signaling, 9040), RPA2 (Abcam, AB2175), pRPA2 S33 (Bethyl Laboratories, A300-246), pRPA2 S4/S8 (Bethyl Laboratories, A300-245), Chk1 (Santa Cruz Biotechnology, SC-8408), and pChk1 (Cell Signaling, 2348). For Western blotting analysis, secondary antibodies HRP-linked anti-rabbit IgG and HRP-linked anti-mouse IgG were purchased from Cell Signaling. For immunofluorescence, Alexa fluor 488 goat anti-rabbit and Alexa fluor 594 goat anti-mouse were used (Invitrogen).

Leung J.W., Makharashvili N., Agarwal P., Chiu L.Y., Pourpre R., Cammarata M.B., Cannon J.R., Sherker A., Durocher D., Brodbelt J.S., Paull T.T, & Miller K.M. (2017). ZMYM3 regulates BRCA1 localization at damaged chromatin to promote DNA repair. Genes & Development, 31(3), 260-274.

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Antibody Validation for Cell Research

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Primary antibodies used in this study were Flag M2 (Sigma, F1804; 1:3000 for blotting), Myc (Santa Cruz, sc-40; 1:1000 for blotting), GFP (Invitrogen, A11122; 1:1000 for blotting), 53BP1 (Novus Biologicals, NB100-304; 1:500 for immunofluorescence), BRCA1 (Santa Cruz Biotechnology, SC-6954; 1:500 for immunofluorescence), γH2AX (Millipore, 05-636; 1:1000 for blotting, 1:500 for immunofluorescence), tubulin (Abcam, AB 6046; 1:5000 for blotting), FK2 (Millipore, 04-263; 1:500 for immunofluorescence), actin (Santa Cruz, sc-1616; 1:5000 for blotting), H2AZ (Cell Signaling, 2718; 1:1000 for blotting), MacroH2A (Abcam, ab37264: 1:1000 for blotting) and MacroH2A2 (Sigma Aldrich, HPA035865; 1:1000 for blotting). For western blotting, secondary antibodies HRP-linked anti-rabbit IgG and HRP-linked anti-mouse IgG were purchased from Cell Signaling (0704 and 0706; 1:1000). For immunofluorescence, Alexa Fluor 488 goat anti-rabbit and Alexa Fluor 594 goat anti-mouse antibodies were used (Invitrogen; 1:500), DAPI (Thermo Fisher Scientific; 1:2000).

Kelliher J.L., West K.L., Gong Q, & Leung J.W. (2020). Histone H2A variants alpha1-extension helix directs RNF168-mediated ubiquitination. Nature Communications, 11, 2462.

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