Methotrexate mtx

Manufactured by Thermo Fisher Scientific

Sourced in United States

Methotrexate (MTX) is a lab equipment product that functions as a chemical compound. It is a folate antagonist used in various applications, including research and clinical settings. The core function of MTX is to inhibit the enzyme dihydrofolate reductase, which is essential for the synthesis of DNA, RNA, and certain proteins.

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Lab products found in correlation

1 2 distearoyl sn glycero 3 phosphoethanolamine n succinyl polyethylene glycol 2000 dspe peg cooh, by Avanti Polar Lipids (2 mentions) 1 2 dipalmitoyl sn glycero 3 phosphocholine dppc, by Avanti Polar Lipids (2 mentions) Cholesterol quantitation kit, by Merck Group (1 mentions)

2 protocols using methotrexate mtx

Methotrexate-Loaded PLGA Nanoparticles

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Poly(d,l-lactide-co-glycolide) acid terminated (PLGA, lactide:glycolide 50:50,
Mw 38 000–54 000), 1-ethyl-3-(3-(dimethylamino)propyl)
carbodiimide (EDC), N-hydroxysuccinimide (NHS), triethylamine
(TEA), low-density lipoproteins (LDL), and a cholesterol quantitation
kit were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methotrexate
(MTX) was bought by AlfaAesar (Haverhill, MA, USA). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[succinyl(polyethylene
glycol)-2000] (DSPE-PEG-COOH), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine
(DSPE_NH2), and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
(DPPC) were purchased from Avanti Polar Lipid (Alabaster, AL, USA).
All reagents and solvents were used without further purification.

Di Francesco V., Gurgone D., Palomba R., Ferreira M.F., Catelani T., Cervadoro A., Maffia P, & Decuzzi P. (2020). Modulating Lipoprotein Transcellular Transport and Atherosclerotic Plaque Formation in ApoE–/– Mice via Nanoformulated Lipid–Methotrexate Conjugates. ACS Applied Materials & Interfaces, 12(34), 37943-37956.

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Synthesis of Methotrexate-Loaded Liposomes

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1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-Hydroxysuccinimide (NHS) and Triethylamine (TEA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methotrexate (MTX) and NH2-PEG (1K) were bought by AlfaAesar (Haverhill, MA, USA). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[succinyl(polyethylene glycol)-2000] (DSPE-PEG-COOH), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE-NH2) and 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were purchased from Avanti Polar Lipid (Alabaster, AL, USA). All reagents and solvents were used without further purification. Cy5 was purchased from Luminoprobe (Hunt Valley, MD, USA).

Di Francesco V., Di Francesco M., Decuzzi P., Palomba R, & Ferreira M. (2021). Synthesis of Two Methotrexate Prodrugs for Optimizing Drug Loading into Liposomes. Pharmaceutics, 13(3), 332.

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