Anti phospho thr196 camkiv

Anti-bassoon (cat# ab82958, Abcam), anti-synaptophysin 1 (cat# 101011, Synaptic Systems), anti-phospho-S9-synapsin (cat# 2311, Cell Signaling Technology), anti-synapsin I (cat# 106103, Synaptic Systems), anti-phospho-Thr196-CaMKIV (cat# Sc-28,443-R, Santa Cruz Biotechnology), anti-CaMKIV (cat# ab3557, Abcam), anti-mCherry (cat# ab167453, Abcam), anti-actin (cat# A4700, Sigma) and 6E10 antibody (cat# SIG-39300, Covance) were used in the experiments.

All chemicals were purchased from Sigma–Aldrich (St Louis, MO, USA) unless otherwise noted. Complete mini EDTA-free, protease inhibitor cocktail tablets were from Roche diagnostics (Mannheim, Germany). Tris/glycine buffer (10X), Tris/glycine/SDS buffer (10X), and Precision Plus Protein All Blue Standards (Catalog #161–0373) were obtained from Bio-Rad (Hercules, CA) and anti-AMPKα from Millipore (Billerica, MA), anti-phospho-Thr172-AMPKα, anti-CaMKIV, and anti-rabbit IgG (H+L) (DyLight 680 Conjugate) from Cell Signaling Technology, Inc (Danvers, MA). Anti- CaMKKβ and anti-CaMKKα were obtained from Abgent INC (San Diego, CA). Anti-CaMKI and anti-phospho-Thr177-CaMKI were obtained from Novus Europe (Cambridge, UK). Anti-phospho-Thr196-CaMKIV was obtained from Santa Cruz Biotechnology, INC (Texas, USA). PI/Sybr-14 (LIVE/DEAD sperm viability kit) was purchased from Molecular Probes (Saint Aubin, France).