Spss package 21

We evaluated the success rate of each diagnostic plane obtained by the inexperienced physician (Observer A). The mean scores of each diagnostic element of 8 diagnostic sections were assessed, and the success rate of each diagnostic element was calculated. Inter- and intra-observer agreement on obtaining 8 diagnostic planes with 5D Heart were evaluated using Cohen’s kappa statistic. Values from 0.61 to 0.80 are considered substantial agreement; 0.81–1.00, near-perfect agreement. 95% confidence intervals (CI) of all statistics were calculated. All statistical analyses described above were performed with IBM SPSS package 21.0 (SPSS Inc., Chicago, IL, USA).

The data were statistically analysed using the statistical software SPSS package 21.0 (IBM Corp, Armonk, NY, USA). All the data were first test for homogeneity of variance by Levene tests. Seed germination morphological characteristics and chemical properties were analysed by three-way analysis of variance (ANOVA), with ambient temperature (T), MT concentration (0, 50 and 100 µM), priming time (12 and 24 h) and germination days as factors. Duncan’s multiple range test was then applied to determine significant differences.