Dfc350fx charge coupled device cameras
Manufactured by Leica camera
The DFC350FX charge-coupled-device (CCD) cameras are designed to capture high-quality digital images. The cameras feature a CCD sensor that converts light into electrical signals, which are then processed and stored as digital image data. The DFC350FX cameras are capable of capturing images with high resolution and color depth, making them suitable for a variety of scientific and industrial applications.
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2 protocols using dfc350fx charge coupled device cameras
1
Myxococcus xanthus Cell Culture and Motility Assay
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M. xanthus cells were grown in liquid 1% CTT medium or on 1% CTT–1.5% agar plates at 32°C (36 (link)). All M. xanthus strains are derivatives of the wild-type strain DK1622 (37 (link)). The M. xanthus strains and plasmids used in this work are listed in Tables 1 and 2 , respectively. The in-frame deletions and insertion mutations were generated as described previously (38 (link)). Kanamycin and oxytetracycline were used at concentrations of 40 and 10 μg/ml, respectively. For motility assays, cells were grown in CTT medium to a density of 7 × 108 cells/ml, harvested, and resuspended in 1% CTT to a calculated density of 7 × 109 cells/ml. Five-microliter aliquots of cell suspensions were placed on 0.5% and 1.5% agar supplemented with 0.5% CTT and incubated at 32°C. After 24 h, colony edges were observed using a Leica MZ8 stereomicroscope or a Leica IMB/E inverted microscope and visualized using Leica DFC280 and DFC350FX charge-coupled-device cameras, respectively. T4P-dependent motility was quantified by determination of the increase in colony diameter in three technical replicates.
Escherichia coli strains were grown in LB broth in the presence of relevant antibiotics (39 ). All plasmids were propagated in E. coli Mach1 [ΔrecA1398 endA1 tonA ϕ80ΔlacZΔM15 ΔlacX74 hsdR(rK− mK+)] unless otherwise stated.
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2
Motility and Development Assays for Myxobacteria
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For motility assays, cells were grown in CTT medium, harvested, and resuspended in 1% CTT to a calculated density of 7 × 109 cells ml−1. Five-microliter aliquots of cell suspensions were placed on 0.5% and 1.5% agar supplemented with 0.5% CTT and incubated at 32 °C. After 24 h, colony edges were observed using a Leica MZ8 stereomicroscope or a Leica IMB/E inverted microscope and visualized using Leica DFC280 and DFC350FX charge-coupled-device cameras, respectively. For development assay, cells were harvested and resuspended in MC7 buffer (10 mM MOPS pH 7.0, 1 mM CaCl2) to a calculated density of 7 × 109 cells ml−1. Twenty microliter aliquots of cells were placed on TPM agar (10 mM Tris-HCl pH 7.6, 1 mM K2HPO4/KH2PO4 pH 7.6, 8 mM MgSO4); for development in submerged culture, 50 μl of the cell suspension were mixed with 350 μl MC7 buffer and placed in an 18 mm diameter microtiter dish. Cells were visualized at the indicated time points using a Leica MZ8 stereomicroscope or a Leica IMB/E inverted microscope and imaged using Leica DFC280 and DFC350FX CCD cameras, respectively. Sporulation levels were determined after development for 120 h in submerged culture as the number of sonication- and heat-resistant spores relative to WT.
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