Subgingival plaque was collected from a periodontitis patient and stained with SYTOX Green (Thermo Fisher Scientific). The eDNA scaffold in the plaque was evaluated via fluorescence microscopy (Zeiss Axio Imager M2 with Zen 2.5 pro software). SYTOX Green was visualized using epifluorescence through a ×100/1.4 Plan Achromat objective at 450–490 nm bandwidth excitation and 500–550 nm bandwidth emission filters. The recovered ligature from the mouse periodontitis model was stained with SYTOX Orange and was visualized similarly but with 538–562 nm bandwidth excitation and 570–640 nm bandwidth emission filter settings.
Zen 2.5 pro software
Manufactured by Zeiss
Sourced in Germany
Zen 2.5 pro software is a comprehensive microscope control and imaging software developed by Zeiss. It provides a user-friendly interface for controlling and configuring Zeiss microscopes, as well as advanced image acquisition, processing, and analysis capabilities.
Automatically generated - may contain errors
Lab products found in correlation
Axio imager m2, by Zeiss (1 mentions)
Sytox green, by Thermo Fisher Scientific (1 mentions)
Milli q system, by Merck Group (1 mentions)
Axiocam camera, by Zeiss (1 mentions)
Axio zoom v16, by Zeiss (1 mentions)
Tween 80, by Merck Group (1 mentions)
Apotome 2.0 microscope, by Zeiss (1 mentions)
Axio observer microscope, by Zeiss (1 mentions)
Cellsens software, by Olympus (1 mentions)
3 protocols using zen 2.5 pro software
1
Evaluating eDNA in Periodontal Plaque
2
Characterization of Soybean Oil Emulsions
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Soybean oil was obtained from Alfa Aesar (Haverhill, MA, USA). Ultrapure water produced by a Milli-Q system (Sigma-Aldrich Canada, Oakville, ON, Canada) was used in all experiments. Span 80 and Tween 80 were purchased from Sigma-Aldrich Canada. Viscosity was measured using a Brookfield DV-III Ultra Programmable Rheometer (Brookfield Engineering, Middleboro, MA, USA) with a CP51 spindle and a 25 mL sample volume. Physical stability includes an invariance of rheological behavior. The rheological properties of an emulsion constitute one of the best means of studying the influence of formulation parameters and manufacturing processes, but also a method of controlling the reproducibility of production and conservation44 (link). Particles were imaged on an Zeiss ApoTome 2.0 microscope with Axio ZoomV.16 and an AxioCam Camera (Zeiss, Oberkochen, Germany) and measured with Zen 2.5 Pro software (Zeiss). Stability and release studies were performed by HPLC using a Shimadzu instrument coupled to a UV detector (Shimadzu Scientific Instruments, Columbia, MD, USA) and a Hypersil PFP 5 μm C18 150 Å 50 × 4.60 mm analytical column (Thermo Scientific, San Jose, CA, USA).
3
Quantitative Analysis of Neuronal Subpopulations
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Images for analysis were acquired using the Zeiss Axio Observer Microscope equipped with an Axiocam 503 B/W monochrome camera (Carl Zeiss, Inc.). Epi-fluorescent z-stack images were taken with 20x objective (NA 0.8) 0.227µm/pixel using ZEN2.5 Pro software (Carl Zeiss, Inc.). Further information regarding equipment and settings can be found in Supplementary Table 5 . Image analysis was performed on five serial 14 µm sections per DRG using the Olympus CellSens Software using the “Count and Measure” feature. Within each subpopulation, only cells with visible nuclei DAPI staining were measured to avoid overcounting duplicate cells. First, the total number of neurons with fluorogold signal were counted. Then, we counted the fluorogold-positive neurons that colocalized with either Nav1.8+ or TH+ immunostaining. Experimenters were blinded to genotype for all image acquisition and analysis. Representative images for publication were acquired using the Olympus FV300RS Confocal Laser Scanning Microscope using the 30X objective (UPLSAPO30x; UPLSAPO N 30X SI OIL, NA 1.05, WD 0.8 mm w/ correction collar) using the 3D Z-stack mode on the Complete Fluoview Acquisition Software.
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