Cobas crp latex hs

Weight and height were measured after a 12-h overnight fast while barefoot and in light clothing to calculate BMI. Fasting blood samples were taken from all donors to measure clinical parameters as follows. The concentrations of serum high-sensitivity C reactive protein (hs-CRP) were measured by a particle-enhanced immunoturbidimetric assay (Cobas CRP [latex] HS, Roche Diagnostics, Mannheim, Germany) on a modular automatic analyzer (Hitachi, Ltd., Tokyo, Japan). Plasma leptin and adiponectin were measured by enzyme-linked immunosorbent assays (ELISA; DuoSet ELISA, R&D Systems Europe, Abingdon, UK), and plasma total and high-density lipoprotein (HDL) cholesterol and triglyceride levels were measured by enzymatic methods (Roche Diagnostics Hitachi, Hitachi Ltd, Tokyo, Japan). Low-density lipoprotein (LDL) cholesterol was calculated by the Friedewald formula. A 75-g oral glucose tolerance test was performed after participants had fasted for 12-h overnight to calculate the insulin resistance index (HOMA-IR). Zygosity was confirmed by genotyping 10 informative genetic markers [31 (link)].

Blood samples were collected following an overnight fast. Whole blood, separated plasma and serum samples were frozen at −80°C until further analysis. Samples were analyzed at the HUSLAB facilities using standardized methods. Concentrations of plasma glucose were measured using the spectrophotometric hexokinase and glucose-6-phosphate dehydrogenase assay (Gluko-quant glucose/hexokinase, Roche Diagnostics, Basel, Switzerland) with a Hitachi Modular automatic analyzer and serum insulin with a time-resolved immunofluorometric assay (Perkin Elmer, Waltham, MA, USA). Fasting plasma total cholesterol, high-density lipoprotein cholesterol (HDL) and triglyceride concentrations were determined using enzymatic methods (Roche Diagnostics Hitachi, Hitachi Ltd, Tokyo, Japan). Low-density lipoprotein (LDL) cholesterol was calculated using the Friedewald formula. Serum high-sensitivity C-reactive protein (hs-CRP) was measured using the particle-enhanced immunoturbidimetric assay (Cobas CRP (Latex) HS, Roche Diagnostics) on a Modular automatic analyzer (Hitachi Ltd, Tokyo, Japan).