Leucine free medium

Manufactured by Atlanta Biologicals

Leucine-free medium is a specialized cell culture medium that does not contain the amino acid leucine. This medium is designed for use in research and scientific applications where the absence of leucine is required, such as in the study of cellular processes or the development of specific cell lines.

Automatically generated - may contain errors

Lab products found in correlation

Leucine, by Merck Group (2 mentions) Hela cell, by American Type Culture Collection (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Actinomycin d, by Merck Group (2 mentions) Cycloheximide (chx), by AG Scientific (2 mentions) Fetal bovine serum (fbs), by Atlas Biologicals (2 mentions) Actinomycin d, by AG Scientific (2 mentions) Leucine, by AG Scientific (2 mentions)

2 protocols using leucine free medium

Serum and Leucine Starvation of HeLa Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HeLa cells (ATCC, Manassas, VA, Cat. # CCL-2) were maintained in culture at 37 °C in a humidified incubator with 5% CO₂ in high glucose Dulbecco's modified Eagle's Medium (DMEM, Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (Atlas Biologicals, Fort Collins, CO) and 1% penicillin-streptomycin (Invitrogen). On the day of the experiment, cells were placed in serum-free medium (DMEM) or leucine-free medium (Atlanta Biologicals, Lawrenceville, GA) for 10 h. After 10 h of starvation, 10% serum or 0.76 mM leucine (Sigma, St. Louis, MO) was re-introduced into the culture medium for the indicated times. For experiments using Actinomycin D (Sigma) or cycloheximide (A.G. Scientific, San Diego, CA), cells were prepared as described above with the exception that either Actinomycin D (2 μg/mL) or cycloheximide (100 μg/mL) was added to the medium for 15 min or 2 min, respectively, prior to the re-introduction of serum or leucine.

Black A.J., Gordon B.S., Dennis M.D., Jefferson L.S, & Kimball S.R. (2016). Regulation of protein and mRNA expression of the mTORC1 repressor REDD1 in response to leucine and serum. Biochemistry and Biophysics Reports, 8, 296-301.

+ Open protocol

+ Expand

Serum and Leucine Starvation Response

Check if the same lab product or an alternative is used in the 5 most similar protocols

HeLa cells (ATCC, Manassas, VA, Cat. # CCL-2) were maintained in culture at 37°C in a humidified incubator with 5% CO₂ in high glucose Dulbecco's modified Eagle's Medium (DMEM, Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (Atlas Biologicals, Fort Collins, CO) and 1% penicillin-streptomycin (Invitrogen). On the day of the experiment, cells were placed in serum-free medium (DMEM) or leucine-free medium (Atlanta Biologicals, Lawrenceville, GA) for 10 hours. After 10 hours of starvation, 10% serum or 0.76 mM leucine (Sigma, St. Louis, MO) was re-introduced into the culture medium for the indicated times. For experiments using Actinomycin D (Sigma) or cycloheximide (A.G. Scientific, San Diego, CA), cells were prepared as described above with the exception that either Actinomycin D (2ug/mL) or cycloheximide (100ug/mL) was added to the medium for 15 min or 2 min, respectively, prior to the re-introduction of serum or leucine.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!