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Leucine free medium

Manufactured by Atlanta Biologicals

Leucine-free medium is a specialized cell culture medium that does not contain the amino acid leucine. This medium is designed for use in research and scientific applications where the absence of leucine is required, such as in the study of cellular processes or the development of specific cell lines.

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2 protocols using leucine free medium

1

Serum and Leucine Starvation of HeLa Cells

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HeLa cells (ATCC, Manassas, VA, Cat. # CCL-2) were maintained in culture at 37 °C in a humidified incubator with 5% CO2 in high glucose Dulbecco's modified Eagle's Medium (DMEM, Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (Atlas Biologicals, Fort Collins, CO) and 1% penicillin-streptomycin (Invitrogen). On the day of the experiment, cells were placed in serum-free medium (DMEM) or leucine-free medium (Atlanta Biologicals, Lawrenceville, GA) for 10 h. After 10 h of starvation, 10% serum or 0.76 mM leucine (Sigma, St. Louis, MO) was re-introduced into the culture medium for the indicated times. For experiments using Actinomycin D (Sigma) or cycloheximide (A.G. Scientific, San Diego, CA), cells were prepared as described above with the exception that either Actinomycin D (2 μg/mL) or cycloheximide (100 μg/mL) was added to the medium for 15 min or 2 min, respectively, prior to the re-introduction of serum or leucine.
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2

Serum and Leucine Starvation Response

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HeLa cells (ATCC, Manassas, VA, Cat. # CCL-2) were maintained in culture at 37°C in a humidified incubator with 5% CO2 in high glucose Dulbecco's modified Eagle's Medium (DMEM, Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (Atlas Biologicals, Fort Collins, CO) and 1% penicillin-streptomycin (Invitrogen). On the day of the experiment, cells were placed in serum-free medium (DMEM) or leucine-free medium (Atlanta Biologicals, Lawrenceville, GA) for 10 hours. After 10 hours of starvation, 10% serum or 0.76 mM leucine (Sigma, St. Louis, MO) was re-introduced into the culture medium for the indicated times. For experiments using Actinomycin D (Sigma) or cycloheximide (A.G. Scientific, San Diego, CA), cells were prepared as described above with the exception that either Actinomycin D (2ug/mL) or cycloheximide (100ug/mL) was added to the medium for 15 min or 2 min, respectively, prior to the re-introduction of serum or leucine.
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