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Fab9332r

Manufactured by R&D Systems
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FAB9332R is a laboratory equipment product. It is designed to perform specific functions in a research and development setting. The core function of this product is provided without interpretation or extrapolation on its intended use.

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Lab products found in correlation

Lsr 2 flow cytometer, by BD (2 mentions) Fab9333g, by R&D Systems (2 mentions) Ic003r, by R&D Systems (2 mentions) Ic003g, by R&D Systems (2 mentions) Mouse serum igg, by Merck Group (2 mentions) Af 488 mouse anti human ace2, by R&D Systems (1 mentions) Lsrfortessa x 20, by BD (1 mentions)

3 protocols using fab9332r

1

Quantifying Human ACE2 Expression

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Cells were blocked with mouse serum IgG (Sigma, 15381) for 10 min at room temperature and then incubated with specific antibodies; AF-647 mouse anti-human ACE2 (Clone # 535919) (R&D systems, FAB9332R), AF-488 mouse anti-human ACE2 (Clone # 171607) (R&D systems, FAB9333G) (0.4 µg/106 cells), AF-647 isotype control mouse IgG2b (Clone # 20102) (R&D systems, IC003R) or AF-488 isotype control mouse IgG2bAF488 (Clone # 20102) (R&D systems, IC003G) for 45 min on ice, followed by washing twice with 2% EV-free FBS/PBS. Finally, the cells were diluted in 2% EV-free FBS/PBS and analyzed on a BD-LSR II flow cytometer (BD Biosciences). Data were analyzed by BD FACSDiva softwares v8.0.2 or v8.0.3 or Flow Jo v10.6.2.
El-Shennawy L., Hoffmann A.D., Dashzeveg N.K., McAndrews K.M., Mehl P.J., Cornish D., Yu Z., Tokars V.L., Nicolaescu V., Tomatsidou A., Mao C., Felicelli C.J., Tsai C.F., Ostiguin C., Jia Y., Li L., Furlong K., Wysocki J., Luo X., Ruivo C.F., Batlle D., Hope T.J., Shen Y., Chae Y.K., Zhang H., LeBleu V.S., Shi T., Swaminathan S., Luo Y., Missiakas D., Randall G.C., Demonbreun A.R., Ison M.G., Kalluri R., Fang D, & Liu H. (2022). Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2. Nature Communications, 13, 405.
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2

Quantifying ACE2 and Cas9 Expression

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To confirm the expression of ACE2 and GFP-tagged Cas9, A549-AC cells were harvested and stained with anti-human ACE2 antibody (FAB9332R, R&D systems, Minneapolis, MN; at the working concentration of 1:100). The expression levels of ACE2 and tagged GFP were determined by an LSRFortessa X-20 (BD Biosciences).
Hou J., Wei Y., Zou J., Jaffery R., Sun L., Liang S., Zheng N., Guerrero A.M., Egan N.A., Bohat R., Chen S., Zheng C., Mao X., Yi S.S., Chen K., McGrail D.J., Sahni N., Shi P.Y., Chen Y., Xie X, & Peng W. (2024). Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infection. Nature Communications, 15, 109.
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3

ACE2 Expression Analysis by Flow Cytometry

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Cells were blocked with mouse serum IgG (Sigma, 15381) for 10 min at room temperature and then incubated with specific antibodies; AF-647 mouse anti-human ACE2 (R&D systems, FAB9332R), AF-488 mouse anti-human ACE2 (R&D systems, FAB9333G), AF-647 isotype control mouse IgG2b (R&D systems, IC003R) or AF-488 isotype control mouse IgG2bAF488 (R&D systems, IC003G) for 45 min on ice, followed by washing twice with 2% exosome-free FBS/PBS. Finally, the cells were diluted in 2% exo-free FBS/PBS and analyzed on a BD-LSR II flow cytometer (BD Biosciences).
El-Shennawy L., Hoffmann A.D., Dashzeveg N.K., Mehl P.J., Yu Z., Tokars V., Nicolaescu V., Ostiguin C., Jia Y., Li L., Furlong K., Mao C., Wysocki J., Batlle D., Hope T.J., Shen Y., Luo Y., Chae Y.K., Zhang H., Swaminathan S., Prescott M., Demonbreun A.R., Ison M.G., Fang D., & Liu H. (2020). Circulating ACE2-expressing Exosomes Block SARS-CoV-2 Infection as an Innate Antiviral Mechanism.
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