The protein for co-immunoprecipitation were collected using a Universal Magnetic Co-IP Kit (Active Motif, CA, USA). To confirm the interaction of endogenous proteins, lysates were precleared with 25 μL Anti-Rabbit IgG Magnetic Beads (Active Motif) for 1 h at 4°C. The supernatant was discarded, and anti-hTERT antibody were incubated with the precleared cell lysates for 4 h at 4°C. The Magnetic Anti-Rabbit IgG Bead complexes were washed three times with IP wash buffer (Active Motif) and eluted in 2 × SDS loading buffer, followed by SDS/PAGE and immunoblotting.
Anti rabbit igg magnetic beads
Manufactured by Active Motif
Anti-Rabbit IgG Magnetic Beads are a laboratory tool used to isolate and purify rabbit immunoglobulin G (IgG) from complex samples. The beads are coated with anti-rabbit IgG antibodies, which bind to the target IgG molecules. The magnetic properties of the beads allow for easy separation and retrieval of the bound IgG using a magnetic field.
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2 protocols using anti rabbit igg magnetic beads
1
Co-immunoprecipitation of Endogenous Proteins
2
Endogenous Protein Co-Immunoprecipitation
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The protein for co-immunoprecipitation were collected using a Universal Magnetic Co-IP Kit (Active Motif, CA, USA). To confirm the interaction of endogenous proteins, lysates were precleared with 25 μL Anti-Rabbit IgG Magnetic Beads (Active Motif) for 1 h at 4°C. The supernatant was discarded, and anti-hTERT and anti-ZEB1 (Abcam) were incubated with the precleared cell lysates for 4 h at 4°C. The Magnetic Anti-Rabbit IgG Bead complexes were washed three times with IP wash buffer (Active Motif) and eluted in 2 × SDS loading buffer, followed by SDS/PAGE and immunoblotting.
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