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Hup t3

Manufactured by Merck Group
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Sourced in United States

The HuP-T3 is a laboratory equipment product manufactured by Merck Group. It is designed to perform specific functions in a laboratory setting. The core function of the HuP-T3 is to [description not available].

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Lab products found in correlation

Hct116, by Thermo Fisher Scientific (1 mentions) Hek293t, by Fujifilm (1 mentions) Hup t3, by Thermo Fisher Scientific (1 mentions) Aspc 1, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Fujifilm (1 mentions) Mccoy s 5a modified medium, by Merck Group (1 mentions) Penicillin streptomycin, by Fujifilm (1 mentions) Hct116, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions) Aspc 1, by American Type Culture Collection (1 mentions) Dld 1, by American Type Culture Collection (1 mentions) Hek293t, by American Type Culture Collection (1 mentions) Transit lt1 transfection reagent, by Mirus Bio (1 mentions) Dmem f12 medium, by Corning (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions)

2 protocols using hup t3

1

Cell Culture and Transfection Protocol

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The human cell lines used in this study include HCT116, DLD-1, AsPC-1, PL5 and HEK293T (American Type Culture Collection, Manassas, VA, USA) and HuP-T3 (Sigma-Aldrich, St Louis, MO, USA). Cell culture was carried out in McCoy's 5A modified medium (Sigma-Aldrich; for HCT116), RPMI-1640 medium (Wako, Osaka, Japan; for DLD-1, AsPC-1 and PL5) and Dulbecco's modified Eagle's medium (Wako; for HuP-T3 and HEK293T). Media were supplemented with 5% fetal bovine serum (BioWest, Nuaillé, France) and 1% penicillin/streptomycin (Wako) prior to use for regular cell culture. The transfection of cells with the plasmids was performed using TransIT-LT1 transfection reagent (Mirus Bio, Madison, WI, USA) according to the manufacturer's instructions. Selection of cells with G418 (Life Technologies) was carried out at concentrations of 0.4 mg/ml (PL5 and HuP-T3), 0.8 mg/ml (HCT116 and AsPC-1) or 1 mg/mL (DLD-1) throughout the study.
Karnan S., Ota A., Konishi Y., Wahiduzzaman M., Hosokawa Y, & Konishi H. (2015). Improved methods of AAV-mediated gene targeting for human cell lines using ribosome-skipping 2A peptide. Nucleic Acids Research, 44(6), e54.
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2

Establishment of Patient-Derived PDAC Models

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Patient-derived samples were obtained from consented patients under an Institutional Review Board (IRB)-approved protocol LAB07-0854 chaired by J.B.F. (UTMDACC). Patient-derived models PATC53, PATC124, PATC148 and PATC153 were generated as previously described5 (link) and kindly provided by Dr Jason Fleming and Dr Michael Kim (MDACC). The cells were routinely maintained in DMEM/F12 Medium (Corning #10-090-CV) supplemented with 10% fetal bovine serum (Sigma #F2442) in a humidified incubator (37 °C, 5% CO2). LSL-KrasG12Dp53L/+ mouse PDAC cell lines were kindly provided by Dr Haoqiang Ying and cultured in RPMI 1640 (Gibco #72400-047) supplemented with 10% fetal bovine serum. PK-59 cell line was obtained from Riken Cell Bank and maintained in RPMI 1640 (Gibco #72400-047) supplemented with 10% fetal bovine serum. KP-3 and KP-4 cell lines were obtained from JCRB Cell Bank. Hup-T3 and Hup-T4 were obtained from Sigma. All other PDAC generic cell lines were obtained from ATCC and maintained following ATCC’s recommendations. Cell lines were validated by STR profiling and confirmed to be negative for mycoplasma.
Giuliani V., Miller M.A., Liu C.Y., Hartono S.R., Class C.A., Bristow C.A., Suzuki E., Sanz L.A., Gao G., Gay J.P., Feng N., Rose J.L., Tomihara H., Daniele J.R., Peoples M.D., Bardenhagen J.P., Geck Do M.K., Chang Q.E., Vangamudi B., Vellano C., Ying H., Deem A.K., Do K.A., Genovese G., Marszalek J.R., Kovacs J.J., Kim M., Fleming J.B., Guccione E., Viale A., Maitra A., Emilia Di Francesco M., Yap T.A., Jones P., Draetta G., Carugo A., Chedin F, & Heffernan T.P. (2021). PRMT1-dependent regulation of RNA metabolism and DNA damage response sustains pancreatic ductal adenocarcinoma. Nature Communications, 12, 4626.
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