Dmem and rpmi 1640 media

TNM A was prepared according to the previous reports.^[19b
] PC, cholesterol, and DSPE‐PEG₂₀₀₀ were purchased from Sigma‐Aldrich (Munich, Germany) or Huateng Pharmaceutical (Changsha, China). DSPE‐PEG₂₀₀₀‐NHS was purchased from Ruixi Biotechnology (Shanxi, China). Trastuzumab was purchased from Roche (Basel, Switzerland). Dialysis bags were purchased from Yuanye Biotechnology (Shanghai, China). Methanol, ethanol, and dichloromethane were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Cell Counting Kit‐8 was purchased from Dongren Chemical Technology Co., Ltd. (Kami Kumamoto, Japan). DMEM and RPMI 1640 media, as well as fetal bovine serum (FBS) and 0.25% (w/v) trypsin solution were purchased from Gibco (Grand Island, NY, USA). Penicillin and streptomycin were purchased from HyClone (Logan, UT, USA). Phosphate buffered saline (PBS) (pH 7.4) was from Gibco (Paiseley, UK).

PLGA 502H, ratio of lactide/glycolide being 50:50 while the inherent viscosity was 0.22 dl/g was obtained from Boheringer Ingelheim, Germany. Poloxamer 188, HCl, NaOH, SDS, DMF and DMSO were procured from Sigma Aldrich, China. DMEM and RPMI 1640 media, FBS, PBS, penicillin, streptomycin, trypsin, EDTA, HBSS were acquired from GIBCO, China. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Propidium Iodide (PI) were acquired from Sigma Aldrich, China. The anti-cancer drugs gemcitabine and epirubicin along with zoledronic acid were from Sun Pharma advanced research centre, India. All the tissue culture and cell plates were procured from NUNC, Finland. Human bone particles were obtained from Merck research laboratories for research use and no approval/clearance was needed for use of this research material, as suggested by the Jilin University Committee on use of Human subjects/materials. No information identifying the subjects was provided by the vendor and no consent was needed for our research study as this was commercially available product without such requirements/limitations.