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Kanamycin sulphate

Manufactured by Carl Roth
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Kanamycin sulphate is a broad-spectrum antibiotic used in laboratory settings. It is a white to off-white powder that inhibits bacterial protein synthesis, making it effective against various microorganisms. Its core function is to serve as a selective agent in cell culture and molecular biology experiments.

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Lab products found in correlation

Ampicillin sodium salt, by Carl Roth (1 mentions) Levofloxacin, by Merck Group (1 mentions) Nalidixic acid, by Merck Group (1 mentions) Penicillin g, by Merck Group (1 mentions) Gentamicin, by Merck Group (1 mentions)

2 protocols using kanamycin sulphate

1

Antimicrobial Compound Acquisition Protocol

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Temporin-L was purchased from CRB Discovery; ampicillin sodium salt and kanamycin sulphate were purchased from Carl Roth; 3,6-bis(dimethylamino)-10-nonyl-acridinium bromide (NAO), gentamicin, penicillin G, levofloxacin, nalidixic acid, and Lisogeny-Broth (LB) medium were purchased from Sigma Aldrich. All other reagents were of molecular biology grade.
Karapetian M., Alimbarashvili E., Vishnepolsky B., Gabrielian A., Rosenthal A., Hurt D.E., Tartakovsky M., Mchedlishvili M., Arsenadze D., Pirtskhalava M, & Zaalishvili G. (2024). Evaluation of the synergistic potential and mechanisms of action for de novo designed cationic antimicrobial peptides. Heliyon, 10(6), e27852.
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2

Membrane Protein Purification Protocol

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Kanamycin sulphate and cefoxitin sodium salt were obtained from Carl Roth (Mannheim, Germany), gentamicin sulphate from Applichem PanReac (Darmstadt, Germany), paromomycin sulphate from Cayman Chemical (Michigan, U.S.A.), amikacin disulphate from Acros Organics (Geel, Belgium) potassium sulphate salt was obtained from Sigma-Aldrich (Dorset, United Kingdom), 1,2-diphytanoyl-sn-glycero-3-phosphocoline was purchased from Avanti Polar Lipids (Alabaster, AL) and all other chemicals used were procured from AppliChem. The proteins were produced in house following previous publications 18 and SI.
Bafna J.A., Sans-Serramitjana E., Acosta-Gutiérrez S., Bodrenko I.V., Hörömpöli D., Berscheid A., Brötz-Oesterhelt H., Winterhalter M, & Ceccarelli M. (2020). Kanamycin Uptake into Escherichia coli Is Facilitated by OmpF and OmpC Porin Channels Located in the Outer Membrane. ACS infectious diseases, 6(7).
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