Anti pstat3

Total proteins from the THP-1 cells treated with MM1.S- and SW480-derived SEVs and from MM1.S- and SW480-derived SEVs were isolated and analyzed by SDS-PAGE followed by Western Blotting. The primary antibodies used in the experiments were as follows: anti-PD-L1 antibody (Abcam, Cambridge, UK, ab213524, diluition 1:1000; negative and positive controls of the antibody are included in Supplementary Figure S5), anti-pSTAT3 (R&D System, AF4607-SP, diluition 1:500), anti-STAT3 (Novus Biologicals, Denver, CO, USA, NBP2-24463, diluition 1:1000), anti-HSP70 (Novus Biologicals, NB600-1469, diluition 1:1000), anti-HSC70 (Santa Cruz, Dallas, TX, USA, sc-7298, diluition 1:500), anti-Calnexin (Santa Cruz, sc-23954, diluition 1:500), anti-Tubulin (Santa Cruz, sc-398103, diluition 1:1000), anti-β Actin (Santa Cruz, sc-81178, diluition 1:1000), anti-GAPDH (Santa Cruz, sc-47724, diluition 1:1000), and anti-NF-kB (Novus, NB100-2176, diluition 1:500). After overnight incubation with the primary antibodies at 4 °C, the membranes were incubated with HRP-conjugated secondary antibody (Thermo Fisher Scientific, Cambridge, MA, USA) for 1 h at 4 °C; the chemiluminescent signal was detected by Chemidoc (Biorad, Milan, Italy).