Bromodeoxyuridine brdu cell proliferation assay kit

Thapsigargin (TG), melatonin, rabbit polyclonal anti-β-actin antibody (catalog number A2066, epitope: amino acids 365–375 of human β-actin), bovine serum albumin (BSA), hydrocortisone (catalog number: H0888), insulin (catalog number: I9278), epidermal growth factor (catalog number: E9644), OAG and cholera toxin (catalog number: C8052) were from Sigma. Fura-2 acetoxymethyl ester (fura-2/AM) was from Molecular Probes. Rabbit polyclonal anti-TRPC6 antibody (catalog number: ACC-120, epitope corresponding to amino acid residues 573–586) was from Alomone. Rabbit polyclonal antiubiquitin antibody (catalog number: ab19247) was from Abcam. DharmaFECT kb transfection reagent was from Cultek. Mouse monoclonal anti-PMCA antibody (Clone 5F10, epitope: amino acids 724–783 of human PMCA) and Live/Dead viability/cytotoxicity kit were from Thermo Fisher. Horseradish-peroxidase-conjugated anti-mouse IgG antibody and anti-rabbit IgG antibody were from Jackson ImmunoResearch Europe Ltd. RNA control vector was from Origene. Enhanced chemiluminescence detection reagents were from Pierce. Bromodeoxyuridine (BrdU) cell proliferation assay kit was from BioVision. SAR7334 was from Quimigen. hTRPC6-YFP was a gift from Craig Montell (Addgene plasmid#21084; http://n2t.net/addgene:21084; RRID:Addgene_21084). All other reagents were of analytical grade.

Fura-2 acetoxymethyl ester (fura-2/AM) was purchased from Molecular Probes (Leiden, The Netherlands). Thapsigargin (TG), a rabbit polyclonal anti-β-actin antibody (catalog number A2066, epitope: amino acids 365–375 of human β-actin), bovine serum albumin (BSA), insulin (catalog number: I9278), epidermal growth factor (catalog number: E9644), esiOrai3 (a pool of siRNAs that target the Orai3 mRNA), and cholera toxin (catalog number: C8052) were purchased from Sigma-Aldrich (Madrid, Spain). A rabbit polyclonal anti-TMEM66 (SARAF) antibody (catalog number PA5-24237, epitope amino acids 33–62 of the N-terminal region of human TMEM66), and a Live/Dead^® viability/cytotoxicity kit were purchased from Thermo Fisher (Madrid, Spain). DharmaFECT was purchased from Dharmacon (Lafayette, CO, USA). A rabbit polyclonal anti-EFHB antibody (catalog number AP10904a, epitope: amino acids 1–30 of human EFHB (N-terminal)) was purchased from Abgent (San Diego, CA, USA). A horseradish peroxidase-conjugated anti-rabbit IgG antibody was purchased from Jackson (Cambridge, UK). Enhanced chemiluminescence detection reagents were from Pierce (Cheshire, UK). A bromodeoxyuridine (BrdU) cell proliferation assay kit was purchased from BioVision (Milpitas, CA, USA). All other reagents were of analytical grade.