To assess the roles of GABA or SDF-1/CXCL12 signaling on migration of human periventricular endothelial cells, 104 cells were seeded in one well insert and allowed to migrate in the presence of respective agonist or antagonist in periventricular endothelial cell medium (without GABA). After five days, cells were fixed, stained with anti-human CD31 antibody (Millipore), imaged and the distance migrated was calculated using ImageJ. To examine the effect of endothelial GABA or endothelial SDF-1/CXCL12 signaling on interneuron migration, human periventricular endothelial cells were seeded in 35 mm dish (105cells/cm2) and incubated with respective chemical for a period of 48 hours. 3 X 104 GABAergic interneurons were seeded on top of the periventricular endothelial cells using a one-well insert, and allowed to migrate over the pre-incubated periventricular endothelial cells for 2 days. Migration of neurons was assayed by staining with anti-human β-Tubulin antibody (Biolegend) and imaged. The concentration of chemicals used in the assays are as follows: muscimol (Sigma) 100 uM, BMI (Sigma) 100 uM, AMD3100 (Sigma) 50μM, recombinant human SDF-1α (Peprotech) 40 nM. The chemicals were kept at −20° as concentrated stock solutions and diluted on the day of the experiment.
Anti human cd31 antibody
Manufactured by Merck Group
The Anti-human CD31 antibody is a laboratory equipment product used for the identification and analysis of CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1), a cell surface glycoprotein expressed on endothelial cells, platelets, and certain immune cells. This antibody can be utilized in various immunological techniques, such as flow cytometry and immunohistochemistry, to detect and study the presence and distribution of CD31 in biological samples.
Automatically generated - may contain errors
2 protocols using anti human cd31 antibody
1
Periventricular Endothelial Cell Migration
2
Roles of GABA and SDF-1 in Neurovascular Migration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To assess the roles of GABA or SDF-1/CXCL12 signaling on migration of human PVECs, 104 cells were seeded in one-well insert and allowed to migrate in the presence of respective agonist or antagonist in PVEC medium (without GABA). After 5 days, cells were fixed, stained with anti-human CD31 antibody (Millipore), imaged and the distance migrated was calculated using ImageJ. To examine the effect of endothelial GABA or endothelial SDF-1/CXCL12 signaling on interneuron migration, human PVECs were seeded in 35-mm dish (105 cells/cm2) and incubated with respective chemical for a period of 48 h. A total of 3 × 104 GABAergic interneurons were seeded on top of the PVECs using a one-well insert, and allowed to migrate over the pre-incubated PVECs for 2 days. Migration of neurons was assayed by staining with anti-human β-tubulin antibody (Biolegend) and imaged. The concentration of chemicals used in the assays are as follows: muscimol (Sigma) 100 µM, BMI (Sigma) 100 µM, AMD3100 (Sigma) 50 µM, recombinant human SDF-1α (Peprotech) 40 nM. The chemicals were kept at −20° as concentrated stock solutions and diluted on the day of the experiment.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!