Human osm duoset elisa

Manufactured by R&D Systems

Sourced in United States

The Human OSM DuoSet ELISA is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of human Oncostatin M (OSM) in cell culture supernatants, serum, and plasma. The kit contains the necessary components for the development of a solid-phase sandwich ELISA, including a capture antibody, a detection antibody, and a recombinant human OSM standard.

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Human ELISA DuoSet Human OSM DuoSet ELISA DuoSets ELISAs

2 protocols using human osm duoset elisa

Overexpression of OSMR in Cervical SCC

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We used the representative OSMR overexpressing cervical SCC cell lines CaSki (HPV16-positive) and SW756 (HPV18-positive), in which OSMR was expressed at 3.4- and 4.5-fold greater levels than in a pool of four independent cultures of basal-type normal cervical squamous cells (Ng et al, 2007 (link)). There was no overexpression of OSM in either cell. The cells were obtained from the American Type Culture Collection (ATCC-LGC, Middlesex, UK) and verified by short tandem repeat profiling (Muralidhar et al, 2011 (link)). Cell culture and OSM treatment were performed as described (Winder et al, 2011 (link)). OSM (R&D Systems, Abingdon, UK) was added at 10 ng ml⁻¹, in accordance with previous studies examining long-term effects of OSM in tissue culture (Winder et al, 2011 (link)). OSM secretion into culture medium was measured by sandwich ELISA (human OSM DuoSet ELISA, R&D Systems), according to the manufacturer's instructions.

Kucia-Tran J.A., Tulkki V., Smith S., Scarpini C.G., Hughes K., Araujo A.M., Yan K.Y., Botthof J., Pérez-Gómez E., Quintanilla M., Cuschieri K., Caffarel M.M, & Coleman N. (2016). Overexpression of the oncostatin-M receptor in cervical squamous cell carcinoma is associated with epithelial–mesenchymal transition and poor overall survival. British Journal of Cancer, 115(2), 212-222.

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Quantifying IL31 and OSM Secretion

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To determine the amounts of IL31 and OSM secreted by the cells and in the mouse serum, ELISA tests were performed. Cells were plated at 1 × 10⁶ per 10-cm dish. At confluency following 48 h post miRNA transfections, or treatment with OSM or IL31, culture supernatants were harvested, centrifuged and centrifuged at 10,000 rpm for 10 min, and ELISA of OSM and IL31 was performed using Human OSM DuoSet ELISA and Human IL-31 DuoSet ELISA (R and D Systems, MN, USA) respectively according to the manufacturer’s instructions. Human-specific IL-31 and OSM recombinant protein (R and D Systems, MN, USA) were used as standards to determine the amounts of these cytokines present in the culture supernatants and serum. All assays were performed in triplicate and repeated three times independently.

Parashar D., Geethadevi A., Aure M.R., Mishra J., George J., Chen C., Mishra M.K., Tahiri A., Zhao W., Nair B., Lu Y., Mangala L.S., Rodriguez-Aguayo C., Lopez-Berestein G., Camara A.K., Liang M., Rader J.S., Ramchandran R., You M., Sood A.K., Kristensen V.N., Mills G.B., Pradeep S, & Chaluvally-Raghavan P. (2019). miRNA551b-3p Activates an Oncostatin Signaling Module for the Progression of Triple-Negative Breast Cancer. Cell reports, 29(13), 4389-4406.e10.

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