The bacterial species evaluated in this study were B. animalis (from ACTIVIA ® ), B. longum, (ATCC 15707), B. lactis (LMG 18905), and B. dentium (ATCC 27678); L. acidophilus (ATCC 4356), L. casei (ATCC 393); S. mutans (ATCC 25175 and 3VF2), and S. sobrinus (ATCC 27607). All ATCC strains were obtained from the Oswaldo Cruz Foundation (FIOCRUZ, Rio de Janeiro, RJ, Brazil), and the André Tosello Foundation (Campinas, SP, Brazil). S. mutans 3VF2 is a highly acidogenic clinical strain that was previously characterized and also provided by Dr. Renata de Oliveira Mattos-Graner (FOP-UNICAMP, Piracicaba, Brazil). 9 B. animalis was isolated from ACTIVIA ® yogurt. Bifidobacterium, Lactobacillus, and Streptococcus species were isolated from the following media: transgalactosylated oligosaccharides propionate agar supplemented with lithium mupirocin (50 mg/L) (TOS-MUP agar; Merck Millipore, Darmstadt, Germany), Rogosa Agar (Difco Laboratories, Detroit, USA) and Mitis Salivarius Agar (MSA, Difco), respectively. 7 (link) All bacteria were grown at 37 o C for 48 h in a 5% CO 2 atmosphere.
Rogosa agar
Manufactured by BD
Sourced in Germany, United States
Rogosa Agar is a selective and differential medium used for the isolation and enumeration of lactobacilli from a variety of samples. It is formulated to support the growth of lactobacilli while inhibiting the growth of other microorganisms. The medium contains sodium acetate, which suppresses the growth of most bacteria, while providing a suitable environment for the growth of lactobacilli.
Automatically generated - may contain errors
Lab products found in correlation
S sobrinus, by American Type Culture Collection (1 mentions)
S mutans, by American Type Culture Collection (1 mentions)
L acidophilus, by American Type Culture Collection (1 mentions)
Tos mup agar, by Merck Group (1 mentions)
L casei, by American Type Culture Collection (1 mentions)
B lactis, by American Type Culture Collection (1 mentions)
B longum, by American Type Culture Collection (1 mentions)
Sabouraud dextrose agar, by BD (1 mentions)
Mitis salivarius agar, by BD (1 mentions)
Brain heart infusion broth bhi, by BD (1 mentions)
2 protocols using rogosa agar
1
Evaluation of Probiotic Bacterial Strains
2
Preparation of Oral Microbial Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
Streptococcus mutans (ATCC 25175), Lactobacillus acidophilus (ATCC#IAL-523) and Candida albicans (ATCC 40176) were obtained from Oswaldo Cruz Foundation (FIOCRUZ, Rio de Janeiro, RJ, Brazil). S. mutans and L. acidophilus were cultured on Mitis Salivarius Agar (Difco Laboratories, Detroit, MI, USA) with 0.2 UI bacitracin and Rogosa Agar (Difco Laboratories, Detroit, MI, USA) for 24-48 h at 37°C in 5% CO2. Candida albicans were grown in Sabouraud Dextrose Agar (Difco Laboratories, Detroit, MI, USA) for 24-48 h at 37°C in aerobic conditions. Subsequently, colonies were transferred to Brain-Heart Infusion broth (BHI; Difco Laboratories, Detroit, MI, USA) for 18-24 h at the same conditions. Cultures were adjusted to 1-5x108 cells/mL in order to obtain an inoculum for subsequent tests.
+ Expand
Streptococcus mutans (ATCC 25175), Lactobacillus acidophilus (ATCC#IAL-523) and Candida albicans (ATCC 40176) were obtained from Oswaldo Cruz Foundation (FIOCRUZ, Rio de Janeiro, RJ, Brazil). S. mutans and L. acidophilus were cultured on Mitis Salivarius Agar (Difco Laboratories, Detroit, MI, USA) with 0.2 UI bacitracin and Rogosa Agar (Difco Laboratories, Detroit, MI, USA) for 24-48 h at 37°C in 5% CO2. Candida albicans were grown in Sabouraud Dextrose Agar (Difco Laboratories, Detroit, MI, USA) for 24-48 h at 37°C in aerobic conditions. Subsequently, colonies were transferred to Brain-Heart Infusion broth (BHI; Difco Laboratories, Detroit, MI, USA) for 18-24 h at the same conditions. Cultures were adjusted to 1-5x10
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!