Two human head and neck squamous carcinoma cell lines, Hep-2 and TU212, were purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences (Chinese Academy of Science, Shanghai, China). Cells were cultured in RMPI-1640 medium (Gibco, Waltham, MA,USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Waltham, MA,USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Hyclone, Logan, UT, USA). Cells were cultured at 37 °C in 5%CO2/95% air in a humidified atmosphere. Both cell lines were routinely tested for mycoplasma contamination every 2 months in our lab. Tests for mycoplasma were negative for all duration of the experiment.
Tu212
Manufactured by Cell Resource Center
Sourced in China
The TU212 is a laboratory equipment designed for the analysis and manipulation of cellular samples. It is a compact, automated system that provides a controlled environment for cell culture and experimentation. The core function of the TU212 is to maintain optimal conditions for the growth and maintenance of various cell lines, enabling researchers to conduct their studies in a reliable and consistent manner.
Automatically generated - may contain errors
4 protocols using tu212
1
Culturing Hep-2 and TU212 Cell Lines
2
Cell Culture Protocol for Hep-2 and TU212
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Two human head and neck squamous carcinoma cell lines, Hep-2 and TU212, were purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences (Chinese Academy of Science, Shanghai, China). Both cell lines were routinely tested for mycoplasma contamination every two months in our lab. Tests for mycoplasma were negative for all duration of the experiment. Cells were cultured in RMPI-1640 medium (Gibco, Waltham, MA,USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Waltham, MA,USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Hyclone, Logan, UT, USA). Cells were cultured at 37°C in 5%CO2/95% air in a humidi ed atmosphere.
3
Culturing Head and Neck Squamous Carcinoma Cell Lines
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Two human head and neck squamous carcinoma cell lines, Hep-2 and TU212, were purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences (Chinese Academy of Science, Shanghai, China). Cells were cultured in RMPI-1640 medium (Gibco, Waltham, MA,USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Waltham, MA,USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Hyclone, Logan, UT, USA). Cells were cultured at 37°C in 5%CO2/95% air in a humidi ed atmosphere.
4
LSCC Cell Lines Culturing Protocol
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Two human LSCC cell lines, Hep-2 and TU212 were purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences (Chinese Academy of Science, Shanghai, China). Cells were cultured in RMPI-1640 medium (Gibco, Waltham, MA,USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, Waltham, MA,USA), 100 U/mL penicillin, and 100 µg/mL streptomycin (Hyclone, Logan, UT, USA). Cells were cultured at 37 °C in 5%CO2/95% air in a humidi ed atmosphere.
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