Informed consent was obtained for all specimens collected. The experimental protocol of this study was approved by the Ethics Committee of the Seventh Affiliated Hospital, Sun Yat-sen University. This study abided by the Declaration of Helsinki principles. Immunohistochemical staining for GREM1 (1:100 dilution, Biorbyt, orb10741, Cambridge, UK) was performed on formalin-fixed, paraffin-embedded samples from 55 patients with clinical stage IV CRC. After dewaxing, hydration and antigen repair, the rest of the experimental procedures were performed according to the instructions of the SP Immunohistochemistry Kit (ZSBIO, PV9000, Beijing, China). Finally, after DAB staining, hematoxylin re-staining and neutral resin sealing, the sections were observed under the microscope for the degree of staining.
Sp immunohistochemistry kit
Manufactured by ZSGB-BIO
Sourced in China
The SP Immunohistochemistry Kit is a laboratory equipment product designed for immunohistochemical staining. It provides the essential components required to perform the staining process.
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Lab products found in correlation
2 protocols using sp immunohistochemistry kit
1
Immunohistochemical Analysis of GREM1 in CRC
2
Immunohistochemical Analysis of MLT Receptor
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The MLT kit was purchased from Abcam (Cambridge, UK). Mouse monoclonal MLT receptor antibody (dilution, 1:200; catalog no. ab167108), mouse polyclonal iNOS antibody (dilution, 1:200; catalog no. ab21775) and mouse monoclonal c-fos antibody (dilution, 1:200; catalog no. ab208942) were purchased from Abcam (Cambridge, UK). SP immunohistochemistry kit was purchased from ZSGB-BIO Co., Ltd. (Beijing, China), and the diaminobenzidine (DAB) chromogenic reagent kit was obtained from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China).
Benchtop was purchased from Sunray Borui Experimental Equipment Co., Ltd. (Beijing, China), ELISA kit was purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA), tissue slicing machine was purchased from Leica (Wetzlar, Germany), digital micropipette was purchased from Finnpipette (Vantaa, Finland), micro-ophthalmic surgical instruments were purchased from Suzhou Medical Supplies Factory Co., Ltd. (Suzhou, China), Nikon optical microscope was purchased from Nikon (Tokyo, Japan), and the Image-Pro Plus professional image analysis system (6th edition) was purchased from Media Cybernetics (Rockville, MD, USA).
Benchtop was purchased from Sunray Borui Experimental Equipment Co., Ltd. (Beijing, China), ELISA kit was purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA), tissue slicing machine was purchased from Leica (Wetzlar, Germany), digital micropipette was purchased from Finnpipette (Vantaa, Finland), micro-ophthalmic surgical instruments were purchased from Suzhou Medical Supplies Factory Co., Ltd. (Suzhou, China), Nikon optical microscope was purchased from Nikon (Tokyo, Japan), and the Image-Pro Plus professional image analysis system (6th edition) was purchased from Media Cybernetics (Rockville, MD, USA).
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