Emt6 murine breast carcinoma

EMT6 murine breast carcinoma, LLC murine Lewis lung carcinoma, and RENCA murine renal cell carcinoma cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and were used within 6 months of resuscitation. Cells were routinely tested to be mycoplasma-free. Cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, SigmaAldrich, Rehovot, Israel), and supplemented with 5% fetal calf serum (FCS), 1% L-glutamine, 1% sodium pyruvate, and 1% Pen-Strep-Neomycin in solution, (Biological Industries, Israel). All cells were cultured in a humidified chamber in 5% CO₂ at 37°C.

EMT6 murine breast carcinoma, 4T1 murine mammary adenocarcinoma, and MCF7 human breast carcinoma cell lines were purchased from the American Type Culture Collection, and were used within 6 months after resuscitation. The EMT6-F2 cell line, a metastatic variant of EMT6, was generated in our lab in a similar way that was previously described for MDA-MB-231 human breast carcinoma cells (21 (link)). All cell lines were cultured in Dulbecco's modified Eagle medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum (Biological Industries), 1% L-glutamine, 1% sodium pyruvate, and 1% streptomycin–penicillin–neomycin solution (Biological Industries). Cells were cultured at 37°C in a humidified atmosphere containing 5% CO₂. Some of the cell lines were stably transfected with a GFP-expressing vector (Clontech Laboratories, 632379). Cells were routinely tested to be Mycoplasma-free using EZ-PCR mycoplasma test kit (Biological industries).